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Platelet-derived growth factor-BB-immobilized asymmetrically porous membrane for enhanced rotator cuff tendon healing

  • Original Article
  • Regenerative Medicine
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Rotator cuff tear is a common musculoskeletal disease that often requires surgical repair. Despite of recent advances in surgical techniques, the re-tear rate of the rotator cuff tendon is very high. In this study, a platelet-derived growth factor-BB (PDGF-BB)-immobilized asymmetrically porous membrane was fabricated to investigate the feasibility for enhancing rotator cuff tendon regeneration through the membrane. PDGF-BB is recognized to promote tendon regeneration. The asymmetrically porous membrane was fabricated by polycaprolactone and Pluronic F127 using an immersion precipitation technique, which can allow selective permeability (preventing scar tissue invasion into defect region but allowing permeation of oxygen/nutrients) and incorporation of bioactive molecules (e.g., PDGF-BB) via heparin binding. The PDGF-BB immobilized on the membrane was released in a sustained manner over 42 days. In an animal study using Sprague-Dawley rats, the PDGF-BB-immobilized membrane group showed significantly greater regeneration of rotator cuff tendon in histological and biomechanical analyses compared with the groups of control (suturing) and membrane without PDGF-BB immobilization. The enhancing regeneration of rotator cuff tendon of the PDGF-BB-immobilized membrane may be caused from the synergistic effect of the asymmetrically porous membrane with unique properties (selective permeability and hydrophilicity) as a scaffold for guided tendon regeneration and PDGF-BB sustainedly released from the membrane.

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Authors and Affiliations

  1. Department of Advanced Materials, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Korea

    Hyun Ki Min & Jin Ho Lee

  2. Department of Orthopedic Surgery, The Catholic University of Korea, Daejeon, Korea

    Oh Soo Kwon

  3. Department of Nanobiomedical Science, Dankook University, Cheonan, Korea

    Se Heang Oh

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  1. Hyun Ki Min
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  2. Oh Soo Kwon
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  4. Jin Ho Lee
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Correspondence to Jin Ho Lee.

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Min, H.K., Kwon, O.S., Oh, S.H. et al. Platelet-derived growth factor-BB-immobilized asymmetrically porous membrane for enhanced rotator cuff tendon healing. Tissue Eng Regen Med 13, 568–578 (2016). https://doi.org/10.1007/s13770-016-9120-3

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