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Isolation and identification of mesenchymal stem cells from human mastoid bone marrow

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

A new type of mesenchymal stem cells (MSCs) isolated from mastoid bone marrow obtained during mastoidectomies were identified. The isolated cells were analyzed by fluorescence-activated cell sorting (FACS) using CD45, CD90, CD105, and CD29 for investigation of the characteristics of the isolated cells. Stem cell phenotypes of human mastoid bone marrow-derived cells (hMBMCs) were maintained during passages 0–4. In the proliferation profiles of stem cells, FACS showed positive results for CD90, CD105, CD29, and negative for CD45. We found that the cells differentiated into adipocytes, chondrocytes, osteocytes, neuronal, and epithelial cells. The morphological features of the differentiated cells were also observed. Our results demonstrated that hMBMCs showed general features of MSCs that can differentiate into multiple cell types under specific stimulation, which could be used as a valuable source of MSCs for various applications in tissue engineering and regenerative medicine, especially for otolaryngology such as one stage reconstruction of functional ears.

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Authors and Affiliations

  1. Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea

    Yeon Ju Kim, Seung Gu Park, Hye Jin Lim & Yun-Hoon Choung

  2. Department of Rural and Biosystems Engineering, Chonnam National University, Gwangju, Korea

    Jangho Kim

  3. Chronic Inflammation Disease Research Center, Ajou University School of Medicine, Suwon, Korea

    Chunjie Tian

  4. Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, Korea

    Chunjie Tian

  5. Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, Korea

    Jong Hoon Chung

  6. Department of Medical Sciences, The Graduate School, Ajou University, Suwon, Korea

    Yun-Hoon Choung

  7. Department of Otolaryngology, Ajou University School of Medicine, 164 World cup-ro, Yeongtong-gu, Suwon, 443-380, Korea

    Yun-Hoon Choung

Authors
  1. Yeon Ju Kim
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  2. Seung Gu Park
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  3. Jangho Kim
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  4. Hye Jin Lim
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  5. Chunjie Tian
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  7. Yun-Hoon Choung
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Corresponding author

Correspondence to Yun-Hoon Choung.

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First two authors contributed equally to this study.

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Kim, Y.J., Park, S.G., Kim, J. et al. Isolation and identification of mesenchymal stem cells from human mastoid bone marrow. Tissue Eng Regen Med 12, 195–202 (2015). https://doi.org/10.1007/s13770-015-0427-2

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  • DOI: https://doi.org/10.1007/s13770-015-0427-2

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