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In vitro co-culture strategies to prevascularization for bone regeneration: A brief update

  • Feature Article
  • Regenerative Medicine
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Vascularization is an important event that generates blood vessels for bone healing and regeneration processes. Vascularization of the engineered bone construct can keep the cells alive by sufficiently supplying nutrient and oxygen and delivering progenitor / stem cells and signaling molecules to the defect site. For this reason there have been extensive research efforts to develop new methodologies, which include development of scaffolds, controlled delivery of signaling molecules, and co-culture systems. Among these, the co-cultures of cells, which involve the cross-talks between vasculogenic cells and osteoprogenitor cells, have recently shown to be an effective prevascularization strategy. Here we briefly update recent key co-culture systems modeled with different culture components and the in vitro and in vivo findings of the prevascularized bone constructs.

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Authors and Affiliations

  1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, Republic of Korea

    Guang-Zhen Jin, Cheol-Min Han & Hae-Won Kim

  2. Department of Nanobiomedical Science and BK21 Plus NBM Global Research Center for Regenerative Medicine, Dankook University Graduate School, Cheonan, 330-714, Republic of Korea

    Guang-Zhen Jin & Hae-Won Kim

  3. Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, 330-714, Republic of Korea

    Cheol-Min Han & Hae-Won Kim

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Jin, GZ., Han, CM. & Kim, HW. In vitro co-culture strategies to prevascularization for bone regeneration: A brief update. Tissue Eng Regen Med 12, 69–79 (2015). https://doi.org/10.1007/s13770-014-0095-7

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