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Cloning and characterization of xylanase in cellulolytic Bacillus sp. strain JMY1 isolated from forest soil

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Abstract

Microbes play an important role in carbon turnover in forest ecosystems by producing polysaccharide-degrading enzymes such as cellulase, xylanase, and β-glucosidase. In the present study, we isolated a bacterial strain producing cellulase and xylanase from the Forest Park at Gyeongnam National University of Science and Technology using LB agar plates containing 0.5 % carboxymethyl cellulose and 0.01 % trypan blue. Based on 16S rRNA sequencing and API analysis, the isolated strain was identified as a Bacillus species and named Bacillus sp. JYM1. The optimal growth temperature of Bacillus sp. JYM1 was 37 °C. The maximal activities of carboxymethyl cellulase (CMCase) and xylanase were obtained after a 24-h cultivation. The optimal pH and temperature were 6.0 and 50 °C for CMCase and 5.0 and 50 °C for xylanase, respectively. The gene responsible for the xylanase activity in Bacillus sp. JYM1 was cloned and expressed in Escherichia coli. The expressed recombinant protein showed similar biochemical properties to the xylanase of Bacillus sp. JYM1. Therefore, our results confirmed that the gene cloned from Bacillus sp. JYM1, herein named Bxyn, encodes xylanase.

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Acknowledgments

This study was supported by Gyeongnam National University of Science and Technology in 2014.

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    Authors and Affiliations

    1. Department of Forest Resources, Gyeongnam National University of Science and Technology, 33 Dongjin-ro, Jinju-si, Gyeongsangman-do, Jinju, 52725, Republic of Korea

      Chong-Kyu Lee, Min-Yeong Jang, Hwa Rang Park, Gab-Chul Choo, Hyun Seo Cho, Sam-Bong Park & Bong-Gyu Kim

    2. Nakdong River Basin Environmental Office, Changwon, 51439, Republic of Korea

      Ki-Cheol Oh

    3. Plant Conservation Division, Korea National Arboretum, Pocheon, 11185, Republic of Korea

      Jong-Bin An

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    1. Chong-Kyu Lee
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    Correspondence to Bong-Gyu Kim.

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    Chong Kyu Lee and Min-Yeong Jang have contributed equally to this work.

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    Lee, CK., Jang, MY., Park, H.R. et al. Cloning and characterization of xylanase in cellulolytic Bacillus sp. strain JMY1 isolated from forest soil. Appl Biol Chem 59, 415–423 (2016). https://doi.org/10.1007/s13765-016-0179-2

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