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A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains

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Abstract

A bacterial strain Paenibacillus polymyxa GS01 was isolated from the interior of the roots of Korean cultivars of ginseng (Panax ginseng C. A. Meyer). The cel44C-man26A gene was cloned from this endophytic strain. This 4,056-bp gene encodes for a 1,352-aa protein which, based on BLAST search homologies, contains a glycosyl hydrolase family 44 (GH44) catalytic domain, a fibronectin domain type 3, a glycosyl hydrolase family 26 (GH26) catalytic domain, and a cellulose-binding module type 3. The multifunctional enzyme domain GH44 possesses cellulase, xylanase, and lichenase activities, while the enzyme domain GH26 possesses mannanase activity. The Cel44C enzyme expressed in and purified from Escherichia coli has an optimum pH of 7.0 for cellulase and lichenase activities, but is at an optimum pH of 5.0 for xylanase and mannanase activities. The optimum temperature for enzymatic activity was 50°C for all substrates. No detectable enzymatic activity was detected for the Cel44C-Man26A mutants E91A and E222A. These results suggest that the amino acid residues Glu91 and Glu222 may play an important role in the glycosyl hydrolases activity of Cel44C-Man26A.

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Acknowledgements

This work was supported by the 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology, Republic of Korea. This work was supported by the Brain Korea21 project in 2006. K.M.C is the recipient of a BK21(GPNGAI) fellowship.

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Authors and Affiliations

  1. Division of Applied Life Science, Gyeongsang National University, Chinju, 660-701, South Korea

    Kye Man Cho, Su Young Hong, Sun Mi Lee, Yong Hee Kim & Goon Gjung Kahng

  2. Research Institute of Life Science, Gyeongsang National University, Chinju, 660-701, South Korea

    Han Dae Yun

  3. Department of Agricultural Chemistry, Sunchon National University, Sunchon, 540-742, South Korea

    Hoon Kim

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  1. Kye Man Cho
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Correspondence to Han Dae Yun.

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Cho, K.M., Hong, S.Y., Lee, S.M. et al. A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains. Appl Microbiol Biotechnol 73, 618–630 (2006). https://doi.org/10.1007/s00253-006-0523-2

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