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Anti-inflammatory and anti-arthritic properties of aqueous extract of Nauclea diderrichii (Rubiaceae) stem bark in rats

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Abstract

Nauclea diderrichii, is a common woody plant in Africa where it is traditionally used for the relief and treatment of multiple pain-related conditions. The aim was to evaluate the inflammatory and anti-arthritic properties of the aqueous extract of N. diderrichii stem bark. Carrageenan and formalin were used to induce inflammation while zymosan A was used to induce monoarthritis in rats. The extract was given orally at doses of 100, 200 and 400 mg/kg. The extract significantly reduced carrageenan-induced edema up to 6 h, with 61.94% (6th hour). The extract induces a significant inhibition of the formalin-induced edema from the 2nd to the 8th hour with 61.50% (2nd hour). In continuous treatment, the activity of the extract remains significant from the 2nd to the 10th day of treatment with 63.85% (9th day). The extract (400 mg/kg) induces a significant decrease of the organ weight while the body weight increases significantly. At 400 mg/kg, the extract significantly increases the levels of red blood cells, hemoglobin and proteins, as well as the catalase and SOD activities, then a significant decrease of the levels of white blood cells, lymphocytes, hematocrit, MDA and NO. Furthermore, the extract significantly inhibited zymosan A-induced edema from the 1st to the 5th day with 60.99% (5th day). The joints of the treated animals show a close to normal architecture compared to that of the untreated animals. These results demonstrate the anti-inflammatory and anti-arthritic properties of N. diderrichii and confirm its traditional use to relieve pain and inflammation.

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Authors and Affiliations

  1. Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon

    Marius Mbiantcha, Marcelle Bejoutance Djami, Gilbert Ateufack, Gonzal Eric Tsafack, William Nana Yousseu, Flore Stéphanie Nguemnang Djuichou, Vanessa Marthe Mba Matah, Liliane Kamdem Kenmogne, Flore Carine Adjouzem, Linda Fagni Zenab Ndjoya & Giresse Lambou Tiokeng

  2. Laboratory of Animal Physiology, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaoundé, Cameroon

    Donatien Albert Atsamo

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  1. Marius Mbiantcha
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Contributions

MM and DBM collected plant material, performed pharmacological assays, statistical analysis and prepared the manuscript. DBM, TEG, NYW, DNSF, MMVM, KKL, ACF, ZLFN and TLG conducted the work, collected and analysed the data. MM conceived, revised the manuscript and supervised the study. AG and AAD helped in raw data analysis and provided punctual assistance. All authors critically read and approved the final manuscript for submission.

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Correspondence to Marius Mbiantcha.

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Ethical statement

Experimental protocols used in this study were approved by the laboratory committee (Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang-Cameroon) according to the standard ethical guidelines for Laboratory animal use and care as described in the European Community guidelines; EEC Directive 86/609 / EEC, of the 24th November 1986.

Conflict of interest

Mbiantcha Marius has no conflict of interest. Djami Bejoutance Marcelle has no conflict of interest. Ateufack Gilbert has no conflict of interest. Atsamo Albert Donatien has no conflict of interest. Tsafack Eric Gonzal has no conflict of interest. Nana Yousseu William has no conflict of interest. Djuichou Nguemnang Stéphanie Flore has no conflict of interest. Matah Marthe Vanessa Mba has no conflict of interest. Kenmogne Kamdem Liliane has no conflict of interest. Adjouzem Carine Flore has no conflict of interest. Zenab Linda Fagni Ndjoya has no conflict of interest. Tiokeng Lambou Giresse has no conflict of interest.

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Mbiantcha, M., Djami, M.B., Ateufack, G. et al. Anti-inflammatory and anti-arthritic properties of aqueous extract of Nauclea diderrichii (Rubiaceae) stem bark in rats. ADV TRADIT MED (ADTM) 20, 199–212 (2020). https://doi.org/10.1007/s13596-020-00446-6

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