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Establishment of embryogenic cultures and determination of their bioactive properties in Rosa rugosa

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  • Tissue Culture/Biotechnology
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Abstract

Somatic embryogenesis represents a powerful in vitro plant culture system and provides high potential for the production of biomass in woody plants. Rosa rugosa is known not only for its ornamental value, but also as a source of antioxidants with the potential to be used as ingredients for industrial products such as cosmetics. The present study was conducted to investigate the effects of hormonal concentration on embryogenic callus (EC) induction from petal and leaf segments of this species. The effect of selected cell lines on the production of total phenolics and flavonoids in R. rugosa was also studied. The highest percentage of EC (14.1 ± 3.9%) and root formation (20.3 ± 6.9%) was observed in the presence of 45.0 μM of 2,4-D. Among the three studied lines, the highest total phenolics and flavonoid levels were observed in H-1 (17.71 mg·g-1 DW), while H-18 contained the lowest. Histological studies of the H-1 and H-18 cell lines revealed differences in polyphenol accumulation and polysaccharides. The effect of developmental stage on growth, shoot, and root regeneration, as well as on phenolic and flavonoid content, was also studied in H-18. The present study indicates that selection of the proper type and concentration of hormone is essential for EC induction from petals in R. rugosa. Moreover, the accumulation of bioactive compounds was found to greatly depend on the genotype. The results of this study are useful for the production of selected antioxidants by somatic embryos from R. rugosa petals.

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Literature Cited

  • Bandyopadhyay S, Hamill JD (2000) Ultrastructural studies of somatic embryos of Eucalyptus nitens and comparisons with zygotic embryos found in mature seeds. Ann Bot 86:237–244

    Article  Google Scholar 

  • Cangahuala-Inocente GC, Steiner N, Santos M, Guerra MP (2004) Morphohistological analysis and histochemistry of Feijoa sellowiana somatic embryogenesis. Protoplasma 224:33–40

    CAS  PubMed  Google Scholar 

  • Chen JR, Wu L, Hu BW, Yi X, Liu R, Deng ZN, Xiong XY (2014) The influence of plant growth regulators and light quality on somatic embryogenesis in China rose (Rosa chinensis Jacq.). J Plant Growth Regul 33:295–304

    Article  CAS  Google Scholar 

  • Cho EJ, Yokozawa T, Rhyu DY, Kim HY, Shibahara N (2003a) The inhibitory effects of 12 medicinal plants and their component compounds on lipid peroxidation. Am J Chin Med 31:907–917

    Article  CAS  PubMed  Google Scholar 

  • Cho EJ, Yokozawa T, Rhyu DY, Kim SC, Shibahara N, Park JC (2003b) Study on the inhibitory effects of Korean medicinal plants and their main compounds on the l, l-diphenyl-2-picrylhydrazyl radical. Phytomedicine 10:544–551

    Article  CAS  PubMed  Google Scholar 

  • Czyzowska A, Klewicka E, Pogorzelski E, Nowak A (2015) Polyphenols, vitamin C and antioxidant activity in wines from Rosa canina L. and Rosa rugosa Thunb. J Food Composit Anal 39:62–68

    Article  CAS  Google Scholar 

  • Fehér A, Pasternak T, Dudits D (2003) Transition of somatic plan cells to an embryonic state. Plant Cell Tiss Org 74:201–228

    Article  Google Scholar 

  • Fehér A, Pasternak T, Miskolczi P, Ayaydin F, Dudits D (2001) Induction of the embryogenic pathway in somatic plant cells. Acta Hortic 560:293–298

    Article  Google Scholar 

  • Fehér A, Pasternak T, Otvos K, Miskolczi P, Dudits D (2002) Induction of embryogenic competence in somatic plant cells: a review. Biologia 51:5–12

    Google Scholar 

  • Fiuk A, Rybczynski JJ (2008) Genotype and plant growth regulatordependent response of somatic embryogenesis from Gentiana spp. leaf explants. In Vitro Cell Dev-Pl 44:90–99

    Article  CAS  Google Scholar 

  • Folin C, Ciocalteu V (1927) Tyrosine and tryptophan determination in protein. J Biol Chem 73:627–650

    CAS  Google Scholar 

  • Helsper JPFG, De Vos CHR, Maas FM, Jonker HH, van den Broeck HC, Jordi W, Pot CS, Keizer LCP, Schapendonk AHCM (2003) Response of selected antioxidants and pigments in tissues of Rosa hybrida and Fuchsia hybrida to supplemental UV-A exposure. Physiol Plant 117:171–187

    Article  CAS  Google Scholar 

  • Hill GP (1967) Morphogenesis of shoot primordia in cultured stem tissue of a garden rose. Nature 216:596–597

    Article  Google Scholar 

  • Hsia CN, Korban SS (1996) Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrid and Rosa chinensis Minima. Plant Cell Tiss Org 44:1–6

    Article  CAS  Google Scholar 

  • Junaid A, Mujib A, Bhat MA, Sharma MP (2006) Somatic embryo proliferation, maturation and germination in Catharanthus roseus. Plant Cell Tiss Org 84:325–332

    Article  Google Scholar 

  • Khan MMR, Rahman MM, Islam MS, Begum SA (2006) A simple UV spectrophotometric method for the determination of vitamin C content in various fruits and vegetables at Sylhet area in Bangladesh. J Biol Sci 6:388–392

    Article  CAS  Google Scholar 

  • Kim SW, Oh MJ, Liu JR (2009) Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose. Plant Biotechnol Rep 3:199–203

    Article  Google Scholar 

  • Kunitake H, Imamizo H, Mii M (1993) Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thunb.). Plant Sci 90:187–194

    Article  CAS  Google Scholar 

  • Li X, Krasnyanski SF, Korban SS (2002) Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa. J Plant Physiol 159:313–319

    Article  CAS  Google Scholar 

  • Liangxiong X, Youwei Z, Gang L, Yonghong P (2005) The antioxidant activities and their relationship with the relative polyphenols and flavonols contents of several flowers extracts. Chinese Wild Plant Res 24:51–54

    Google Scholar 

  • Lotito SB, Frei B (2006) Consumption of flavonoid-rich foods and increased plasma antioxidant capacity in humans: cause, consequence, or epiphenomenon. Free Radical Biol Med 41:1727–1746

    Article  CAS  Google Scholar 

  • Misra N, Misra P, Datta SK, Mehrotra S (2005) In vitro biosynthesis of antioxidants from Hemidesmus indicus R. Br. cultures. In Vitro Cell Dev-Pl 41:285–290

    Article  CAS  Google Scholar 

  • Olech M, Nowak R, Los R, Rzymowska J, Malm A, Chrusciel K (2012) Biological activity and composition of teas and tinctures prepared from Rosa rugosa Thunb. Central Eur J Biol 7:172–18

    CAS  Google Scholar 

  • Park HJ, Nam JH, Jung HJ, Lee MS, Lee KT, Jung MH, Choi JW (2005) Inhibitory effect of euscaphic acid and tormentic acid from the roots of Rosa rugosa on high fat diet-induced obesity in the rat. Korean J Pharmacogn 36:321–331

    Google Scholar 

  • Park JA, Park BJ, Kim AH, Park SY, Paek KY (2015) Airlift bioreactor system and nitrogen sources for biomass and antioxidant compound production from in vitro culture of Vitis flexuosa plantlets. Hortic Environ Biotechnol 56:358–365

    Article  CAS  Google Scholar 

  • Pasternak T, Prinsen E, Ayaydin F, Miskolczi P, Potters G, Asard H, Van Onckelen H, Dudits D, Feher A (2002) The role of auxin, pH and stress in the activation of embryogenic cell division in leaf protoplast-derived cells of alfalfa (Medicago sativa L.). Plant Physiol 129:1807–1819

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Scalzo J, Politi A, Pellegrini N, Mezzetti B, Battino B (2005) Plant genotype affects total antioxidant capacity and phenolic contents in fruits. Nutrition 21:207–213

    Article  CAS  PubMed  Google Scholar 

  • Tripathi L, Tripathi JN (2003) Role of biotechnology in medicinal plants. Trop J Pharm Res 2:243–253

    Google Scholar 

  • Tulipani S, Mezzetti B, Capocasa F, Bompadre S, Beekwilder J, Ric de Vos CH, Capanoglu E, Bovy A, Battino M (2008) Antioxidants, phenolic compounds, and nutritional quality of different strawberry genotypes. J Agric Food Chem 56:696–704

    Article  CAS  PubMed  Google Scholar 

  • Vanisree M, Lee CY, Lo CY, Nalawade SM, Lin CY, Tsay SH (2004) Studies on production of some important secondary metabolites from medicinal plants by plant tissue cultures. Bot Bull Acad Sin 45:1–22

    CAS  Google Scholar 

  • Yeung EC (1999) The use of histology in the study of plant tissue culture systems-some practical comments. In Vitro Cell Dev-Pl 35:137–143

    Article  Google Scholar 

  • Youwei Z, Yonghong P (2007) Changes in antioxidant activity in Rosa rugosa flowers at different stages of development. N Z J Crop Hortic Sci 35:397–401

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 28644, Korea

    Hye-Rim Jang, Hyun-Jeong Lee, Kee-Yoeup Paek & So-Young Park

  2. Korea Kolmar, Deokgogaegil 12-11, Jeoneui-myeon, Sejong, 30004, Korea

    Byung-Jun Park

  3. Sejong City Agricultural Technique Center, Sejong, 30048, Korea

    Ok-Ja Pee

Authors
  1. Hye-Rim Jang
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  2. Hyun-Jeong Lee
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  3. Byung-Jun Park
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  4. Ok-Ja Pee
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  5. Kee-Yoeup Paek
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  6. So-Young Park
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Correspondence to So-Young Park.

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Jang, HR., Lee, HJ., Park, BJ. et al. Establishment of embryogenic cultures and determination of their bioactive properties in Rosa rugosa . Hortic. Environ. Biotechnol. 57, 291–298 (2016). https://doi.org/10.1007/s13580-016-0012-1

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  • DOI: https://doi.org/10.1007/s13580-016-0012-1

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