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An efficient callus proliferation protocol and rhaponticin accumulation of Rheum franzenbachii Munt., a medicinal plant

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Abstract

An efficient callus proliferation system for Rheum franzenbachii Munt., a rare medicinal plant, has been developed. Callus induced from leaf explants incubated on Murashige and Skoog (MS) medium with appropriate supplements of plant growth regulators. In the 6-benzylaminopurine (6-BAP) in combination with α-naphthalene acetic acid (NAA) treatments, different concentrations of NAA showed different induction effects on explants. When concentration of 6-BAP was as high as 2.0 mgl−1 in combination with 0.5 mgl−1 NAA, the callus induction rate reached 58.3%. N-phenyl-N’-1,2,3-thiadiazol-5-ylure (TDZ) in combination with NAA was very suitable for callus proliferation compared to TDZ in combination with 2,4-dicholorophenoxy acetic acid (2,4-D) or TDZ in combination with indole-3-acetic acid (IAA). Fresh and dry weight of callus cultured on MS medium supplemented with 0.5 mgl−1 TDZ in combination with 0.2 mgl−1 NAA increased 26.3 and 15.0 times within 35 days culture, respectively. Quantitative analysis of rhaponticin by HPLC showed that the phytochemical profile of callus was similar to that of wild plants, and the content of rhaponticin in callus cultured on MS medium supplemented with 0.5 mgl−1 TDZ and 0.2 mgl−1 NAA was 16.6 mgg−1DW compared to that of 4.0 mgg−1 DW in wild plants.

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Abbreviations

BAP:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

NAA:

Naphthaleneacetic acid

TDZ:

Thidiazuron

2,4-D:

2,4-dicholorophenoxy acetic acid

MS:

Murashige and Skoog

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Acknowledgment

This work was financially supported by National Basic Research Program of China (973 Program, 2009CB522300), the Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China (09708211Z1), Program of State Ethnic Affairs Commission (09ZY09), the “985” Project (MUC985) , “111” Project (B08044) and the Fundamental Research Funds for the Central Universities (0910KYZY46).

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Authors and Affiliations

  1. College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, People’s Republic of China

    Junli Wang, Yuan Lu, Qian Wang, Kun Liu, Yunfei Song & Kaili Bi

  2. College of Biology Science and Engineering, Hebei University of Economics and Business, Shijiazhuang, 050061, People’s Republic of China

    Kun Liu

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  1. Junli Wang
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  2. Yuan Lu
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  3. Qian Wang
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  4. Kun Liu
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Correspondence to Junli Wang.

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Wang, J., Lu, Y., Wang, Q. et al. An efficient callus proliferation protocol and rhaponticin accumulation of Rheum franzenbachii Munt., a medicinal plant. J. Plant Biochem. Biotechnol. 20, 252–257 (2011). https://doi.org/10.1007/s13562-011-0055-4

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