Abstract
The miR-21 detection is essential for the accurate and early diagnosis or prognosis of most diseases. In the current study, the miR-21 detection method was performed based on the exploitation of the simple colorimetric biosensor due to the unique surface plasmon resonance (SPR) absorption of gold nanoparticles. AuNPs were functionalized with the specific DNA probes on the gold surface by Au-SH bond. Two strategies are proposed to detect the miR-21. In the first strategy, probes (DNA1 and DNA2) were complementary to the sequence of the miR-21. When miR-21 is introduced, the hybridization reaction is triggered between the golden nanoparticles- (GNP)-DNA conjugates and targets, resulting in changes in SPR absorption band and macroscopic color of the AuNPs solution. In the second strategy, we used a fluorescence sequence (DNA3-FAM), which was complimentary of DNA1. The DNA3-FAM was released after hybridization of miR-21 with DNA1 and DNA2. The free DNA3-FAM sequences in plate wells containing 1% agarose gel were assessed by fluorescent microscope. The combination of two strategies and conventional equipment such as UV–Vis spectrometers and fluorescent microscopes could be useful in detecting the desired miRNAs (miR-21).
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References
Finotti A, Fabbri E, Lampronti I et al (2019) MicroRNAs and long non-coding RNAs in genetic diseases. Mol Diagnosis Ther 23:155–171. https://doi.org/10.1007/s40291-018-0380-6
Orang AV, Safaralizadeh R, Kazemzadeh-Bavili M (2014) Mechanisms of miRNA-mediated gene regulation from common downregulation to mRNA-specific upregulation. Int. J. Genomics 2014
Orang AV, Safaralizadeh R, Feizi MAH (2014) Insights into the diverse roles of miR-205 in human cancers. Asian Pacific J Cancer Prev 15:577–583
Rupaimoole R, Han H-D, Lopez-Berestein G, Sood AK (2011) MicroRNA therapeutics: principles, expectations, and challenges. Chin J Cancer 30:368–370. https://doi.org/10.5732/cjc.011.10186
Halford WP (1999) (1999) The essential prerequisites for quantitative RT-PCR. Nat Biotechnol 179(17):835–835. https://doi.org/10.1038/12783
B S, SF K, (2002) Measuring proteins on microarrays. Curr Opin Biotechnol 13:14–19. https://doi.org/10.1016/S0958-1669(02)00278-1
Ye J, Xu M, Tian X, et al (2019) Research advances in the detection of miRNA. J Pharm Anal 9:217–226. https://doi.org/10.1016/J.JPHA.2019.05.004
Ta T, Ca M, RL L, (2000) Scanometric DNA array detection with nanoparticle probes. Science 289:1757–1760. https://doi.org/10.1126/SCIENCE.289.5485.1757
Han M, Gao X, Su JZ, Nie S (2001) Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules. Nat Biotechnol 2001 197 19:631–635. https://doi.org/10.1038/90228
Park S-J, Taton TA, Mirkin CA (2002) Array-based electrical detection of DNA with nanoparticle probes. Science (80- ) 295:1503–1506. https://doi.org/10.1126/SCIENCE.1067003
Kim H, Terazono H, Hayashi M, et al (2012) Highly sensitive detection of target biomolecules on cell surface using gold nanoparticle conjugated with aptamer probe. Jpn J Appl Phys 51:06FH01. https://doi.org/10.1143/JJAP.51.06FH01
Li RD, Yin BC, Ye BC (2016) Ultrasensitive, colorimetric detection of microRNAs based on isothermal exponential amplification reaction-assisted gold nanoparticle amplification. Biosens Bioelectron 86:1011–1016. https://doi.org/10.1016/j.bios.2016.07.042
Kira A, Matsuo K, Nakajima S (2015) DNA hybridization activity of single-stranded DNA-conjugated gold nanoparticles used as probes for DNA detection. Jpn J Appl Phys 55:02BE04. https://doi.org/10.7567/JJAP.55.02BE04
Lee H, Park JE, Nam JM (2014) Bio-barcode gel assay for microRNA. Nat Commun 5:3367. https://doi.org/10.1038/ncomms4367
A K, H K, K Y (2009) Contribution of nanoscale curvature to number density of immobilized DNA on gold nanoparticles. Langmuir 25:1285–1288. https://doi.org/10.1021/LA803385X
and SKG, Tarasankar Pal* § (2007) Interparticle coupling effect on the surface plasmon resonance of gold nanoparticles: from theory to applications. Chem Rev 107:4797–4862. https://doi.org/10.1021/CR0680282
Cai J, Ding L, Gong P, Huang J (2019) A colorimetric detection of microRNA-148a in gastric cancer by gold nanoparticle–RNA conjugates. Nanotechnology 31:095501. https://doi.org/10.1088/1361-6528/AB55B7
E C, MA T, S R, et al (2012) MicroRNA conjugated gold nanoparticles and cell transfection. Anal Chem 84:26–29. https://doi.org/10.1021/AC202749P
Huang J, Shangguan J, Guo Q et al (2019) Colorimetric and fluorescent dual-mode detection of microRNA based on duplex-specific nuclease assisted gold nanoparticle amplification. Analyst 144:4917–4924. https://doi.org/10.1039/C9AN01013K
Moros M, Kyriazi M-E, El-Sagheer AH et al (2018) DNA-coated gold nanoparticles for the detection of mRNA in live Hydra vulgaris animals. ACS Appl Mater Interfaces 11:13905–13911. https://doi.org/10.1021/ACSAMI.8B17846
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Zare, N., Ghasemi, R., Rafiee, L. et al. Assessment of microRNA-21 using gold nanoparticle-DNA conjugates based on colorimetric and fluorescent detection. Gold Bull 55, 107–114 (2022). https://doi.org/10.1007/s13404-022-00308-w
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DOI: https://doi.org/10.1007/s13404-022-00308-w