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Recent Advances in Ultrasensitive miRNA Biomarkers Detection

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Advanced Sensors for Biomedical Applications

Part of the book series: Smart Sensors, Measurement and Instrumentation ((SSMI,volume 38))

Abstract

Recently, microRNAs gain a great interest in the bio-molecular field due to their fundamental role in clinical diagnostics. In this chapter, we generally discussed the biogenesis of microRNAs and the progress made for their detection. Researchers have widely tried to investigate their effort to build a sensitive, selective, and accurate platform for microRNA detection. To date, multiple techniques have been developed, ranging from the old conventional method (northern blot, RT-PCR, microarrays) to the newly established ones (biosensors, nanopores). However, given the various challenges related to miRNA detection, such as low abundance, small size, and high level of sequence similarity, different enzymatic and non-enzymatic amplification approaches were successfully exploited to improve such devices’ sensitivity. Among these strategies, HCR, RCA, nanomaterials, and the use of enzyme-based target recycling like DSN enzyme. On the other hand, the combination of different methods has emerged as an ideal option for further enhancement of the sensitivity. In the end, knowing that the expression of a single miRNA is not enough to identify one specific disease, it is usually necessary to implant a simultaneous and multiplexed technique for more sophisticated and efficient diagnostic tools.

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Authors and Affiliations

  1. Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing, PR China

    Khouloud Djebbi, Mohamed Bahri, Mohamed Amin Elaguech, Rong Tian, Shi Biao, Chaker Tlili & Deqiang Wang

  2. Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, PR China

    Khouloud Djebbi, Mohamed Bahri, Mohamed Amin Elaguech, Rong Tian, Shi Biao, Chaker Tlili & Deqiang Wang

  3. Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714, PR China

    Khouloud Djebbi, Mohamed Bahri, Mohamed Amin Elaguech, Rong Tian, Shi Biao, Chaker Tlili & Deqiang Wang

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  1. Khouloud Djebbi
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  2. Mohamed Bahri
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  3. Mohamed Amin Elaguech
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  4. Rong Tian
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  5. Shi Biao
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  6. Chaker Tlili
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  7. Deqiang Wang
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Correspondence to Chaker Tlili or Deqiang Wang .

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Editors and Affiliations

  1. Fakultät für Elektrotechnik und Informationstechnik, Technische Universität Chemnitz, Chemnitz, Germany

    Olfa Kanoun

  2. Department of Electrical Engineering, Ecole Nationale d’Ingénieurs de Sfax, University of Sfax, Sfax, Tunisia

    Nabil Derbel

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Djebbi, K. et al. (2021). Recent Advances in Ultrasensitive miRNA Biomarkers Detection. In: Kanoun, O., Derbel, N. (eds) Advanced Sensors for Biomedical Applications. Smart Sensors, Measurement and Instrumentation, vol 38. Springer, Cham. https://doi.org/10.1007/978-3-030-71225-9_9

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