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Valorization of shrimp waste by obligate alkaliphilic Bacillus marmarensis

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Abstract

Food wastes are one of the great deals of ever-growing world population. Among them, chitinous wastes are precious for being used as a substrate to produce value-added commodities. In the present work, the deproteinization of shrimp waste together with the production of alkaline protease enzyme by obligate alkalophilic Bacillus marmarensis was investigated. First, we determined optimum conditions including inoculum ratio, temperature, shrimp waste concentration, and fermentation time for alkaline protease production. The highest protease activity (269.48 ± 2.19 U/mL) was obtained with 5% sole shrimp waste pretreated by heating at 100 °C for 20 min at 35 °C and pH 10.0 with inoculum ratio of 6%. Under optimized conditions, B. marmarensis showed a deproteinization capacity of 70.62% at pH 10.0 and 56.52% at pH 12.0, respectively, and the Fourier transform infrared spectroscopy spectrum of chitin obtained from deproteinization by B. marmarensis presented the characteristics of chitin compared to literature. Furthermore, the production of alkaline proteases was performed by using different carbon and nitrogen sources in comparison with the enzyme produced solely from shrimp waste. Among the carbon and nitrogen sources used, the highest activity was obtained from sucrose together with peptone from meat as 239.87 ± 28.35 U/mL, which was lower than the activity obtained from sole shrimp waste. Thus, this work indicates that obligate alkalophilic B. marmarensis can be used to develop sustainable bioprocess to produce alkaline proteases from sole shrimp waste and deproteinization of it. To our knowledge, this is the first work for the valorization of shrimp waste by obligate alkalophilic bacteria.

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Funding

The authors acknowledge the financial support received from Marmara University, Scientific Research Project Unit with the project number FYL-2022–10331.

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  1. Faculty of Engineering, Department of Bioengineering, Marmara University, Maltepe, 34854, Istanbul, Turkey

    Merve Canpulat, Orkun Pinar, Zeynep Yilmaz-Sercinoglu & Dilek Kazan

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Contributions

Investigation: Merve Canpulat, writing—original draft preparation: Merve Canpulat, Orkun Pinar, Zeynep Yilmaz-Sercinoglu, Dilek Kazan, validation: Merve Canpulat, visualization: Orkun Pinar, writing—reviewing and editing: Orkun Pinar, Zeynep Yilmaz-Sercinoglu, Dilek Kazan, formal analysis: Orkun Pinar, Dilek Kazan, resources: Dilek Kazan, project administration: Dilek Kazan, conceptualization: Merve Canpulat, methodology: Orkun Pinar, Dilek Kazan, funding acquisition: Dilek Kazan, supervision: Zeynep Yilmaz-Sercinoglu, Dilek Kazan.

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Correspondence to Dilek Kazan.

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Canpulat, M., Pinar, O., Yilmaz-Sercinoglu, Z. et al. Valorization of shrimp waste by obligate alkaliphilic Bacillus marmarensis. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-022-03684-7

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