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A biological clean processing approach for the valorization of speckled shrimp Metapenaeus monoceros by-product as a source of bioactive compounds

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Abstract

The efficiency of the proteolytic strain Anoxybacillus kamchatkensis M1V in the fermentation of speckled shrimp by-product was investigated for the recovery of a deproteinized bioactive hydrolysate. The biological activities of the resulting hydrolysate were also examined by applying several antioxidant and enzyme inhibitory assays. The strain M1V was found to produce high level of protease activity (2000 U/mL) when grown in media containing only shrimp powder at 25 g/L. The crude protease displayed a significant deproteinization capabiliy, with the best efficiency (48%) being recorded for an enzyme to substrate (E/S) ratio of 30 U/mg. Following the deproteinization, chitin was recovered and the authenticity was confirmed by Fourier-transform infrared spectroscopy (FTIR) analysis. On the other hand, the obtained hydrolysate showed a significant enzymatic inhibitory potential against acetylcholinesterase, tyrosinase, amylase, and angiotensin I convertase, and a strong antioxidant activity.

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Acknowledgments

The authors would like to thank Mrs. M. Mezghani Abid, and Mr. A. Hadj Brahim (LMBEB-CBS). Also, the authors were grateful to Mr. I. Hssairi, Mr. A. Zitoun, and Mr. F. Boukhili (Specialized unit, UVRR-CBS) for their technical assistance. Special thanks are also due Dr. Z. Bouallagui from the Centre of Biotechnology of Sfax (Sfax, Tunisia) and Pr. W. Hariz from the English Department at the Sfax Faculty of Sciences, University of Sfax, Tunisia, for constructive proofreading and language polishing services.

Funding

This study was supported by the Ministry of Higher Education and Scientific Research of Tunisia, under the Contract Program 2015-2019: grant no. LMBEE_CBS/code: LR15CBS06 and the Tunisian-Algerian project JAOUADI/BADIS_TNDZ-MicrooZymes_2012-2018/code: TA/04/2012.

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Author notes

  1. Sondes Mechri and Imen Sellem contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia

    Sondes Mechri, Khelifa Bouacem, Fadoua Jabeur & Bassem Jaouadi

  2. Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia

    Imen Sellem & Lotfi Mellouli

  3. Laboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences (FSB), University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria

    Khelifa Bouacem, Hocine Hacène & Amel Bouanane-Darenfed

  4. Laboratory of Functional Analysis of Chemical Processes (LFACP), Process Engineering Department, Faculty of Technology, University of Blida 1, Road of Soumaâ, P.O. Box 270, 09000, Blida, Algeria

    Hassiba Laribi-Habchi

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  1. Sondes Mechri
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  2. Imen Sellem
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Mechri, S., Sellem, I., Bouacem, K. et al. A biological clean processing approach for the valorization of speckled shrimp Metapenaeus monoceros by-product as a source of bioactive compounds. Environ Sci Pollut Res 27, 15842–15855 (2020). https://doi.org/10.1007/s11356-020-08076-w

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