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Simultaneous production of cellulases, hemicellulases, and reducing sugars by Pleurotus ostreatus growth in one-pot solid state fermentation using Alstroemeria sp. waste

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Abstract

This study describes the cellulases (CLS), hemicellulases (HCS), and reducing sugars (RS) production by Pleurotus ostreatus PLO6 growth under solid state fermentation (SSF) using stems and leaves mixture of Alstroemeria sp. (AW) as lignocellulosic (LC) substrate (also named biomass). SSF involved, at the same time, CLS and HCS biosynthesis, and its subsequent action over the substrate in order to obtain RS, which are considered as precursors of bioethanol and other bioproducts. The first stage of this research was performed to verify simultaneous obtaining of hemicellulolytic and cellulolytic enzymes, and RS by means of univariate experimentation (response variables: hemicellulolytic and cellulolytic enzyme activities and RS concentration, factor: time). In this preliminary study, P. ostreatus PLO6 was able to synthesize endoglucanases, exoglucanases, β-glucosidases, endoxylanases, β-xylosidases, and RS concurrently. Afterwards, temperature (T), substrate concentration (SC), and time (t) effects on simultaneous obtaining of hemicellulolytic and cellulolytic enzymes and RS were assessed. A statistical factorial design 23 was developed using the same response variables indicated at the preliminary phase. With a significance level of 0.05, temperature was the most significant effect on enzymatic activities (excepting β-xylosidases) and RS production. CLS activities and RS production were maximized at 24 °C, while HCS activities were better at 32 °C. The SC was statistically significant too, when it was maintained at 30%, the CLS (excepting endoglucanases) and HCS production was favorable. The RS highest production was of 44.8 mg·g−1 (respective to raw AW) and 124.3 mg·g−1 (respective to cellulose) at 24 °C, 20% of SC and 8 days of SSF.

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Acknowledgements

The authors are grateful to the National Council for Scientific and Technological Development (CNPq), Studies and Projects Funding Agency (FINEP), Research Supporting Foundation of Minas Gerais State (FAPEMIG), Improvement Coordination of Higher Education Personnel (CAPES) and Organization of American States (OAS) for their financial support.

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Authors and Affiliations

  1. Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, 38408-902, Brazil

    Hernán Darío Zamora Zamora, Thiago A. L. Silva, Leandro H. R. Varão & Daniel Pasquini

  2. Agricultural Sciences Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, 38405-902, Brazil

    Milla A. Baffi

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  1. Hernán Darío Zamora Zamora
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Correspondence to Daniel Pasquini.

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Highlights

–Simultaneous synthesis of cellulases, hemicellulases, and reducing sugars using one-pot solid state fermentation

–First assessment of Alstroemeria sp. waste as substrate for concurrent production of reducing sugars, cellulases, and hemicellulases

–Obtaining of reducing sugars without pretreatment stage

–Pleurotus ostreatus PLO6 was able to synthesize endoglucanases

Statement of novelty

In relation to valorize vegetable biomass wastes and to avoid their disposal in soil or landfill, this research is the first one performed in Brazil that assessed simultaneous production of hemi(cellulolytic) enzymes (CLS and HCS) and RS from AW by Pleurotus ostreatus PLO6 growth using solid state fermentation (SSF). In addition, this research is pioneer in physical–chemical characterization of Brazilian flower waste and, from biological and chemical viewpoints, in harnessing this kind of waste to obtain CLS, HCS, and RS concurrently. Monosaccharides contained in RS, analyzed by this research, have a starring role in different industries due to they are potential precursors for biofuels and others bioproducts obtaining.

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Zamora Zamora, H.D., Silva, T.A.L., Varão, L.H.R. et al. Simultaneous production of cellulases, hemicellulases, and reducing sugars by Pleurotus ostreatus growth in one-pot solid state fermentation using Alstroemeria sp. waste. Biomass Conv. Bioref. 13, 4879–4892 (2023). https://doi.org/10.1007/s13399-021-01723-3

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