Abstract
A locally soil-isolated strain of Trichoderma citrinoviride produced cellulase activity levels quite similar to the overproducing mutant T. reeSei (T. longibrachiatum) QM 9414 when grown in homogeneous cultures containing sugarcane straw. It was confirmed than the crude enzymatic extracts (filter paper enzyme, carboxymethylcellulase and ß-glucosidase) have similar traits to the reference strain. Both strains utilized more efficiently the ground substrate in relation to other pre-treatments assayed. Kinetics of growth in solid beds containing sugarcane straw showed than the fermentation stopped around 96–120 hours, obtaining the highest enzyme production (filter paper enzyme: 16 U/g ), and released a minimum of residual sugar (glucose and xylose, 5.032 mg/g1 ). The final product is characterized by a reduction in fibre content of the substrate (40.4 %) and an increase in cell protein (6.2 - 7.9 %).
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Alam, M.,Gómez, I.,Mohiuddin, G.and Hoq, M. (1994). Production and characterization of thermostable xylanase byThermomyces lanuginosus andThermoascus aurantiacus growth on lignocelulose.Enz. Microb. Technol.,16(4):298–303.
Colilla F.J. (1996).Condiciones cromatográficas del HPLC para la determinación de azúcares, alcoholes y ácidos orgánicos. Protocolo 29, Planta Piloto de Fermentación, Smithkline, Beecham S.A. Madrid, España.
De la Mata I (1991). α-glucosidasa deTrichoderma reeSei QM 9414. PhD Thesis dissertation. Fac. Biologia, Universidad Complutense de Madrid, España.
Dustet, J.C. (1999). Contribución al estudio y al desarrollo de la fermentación en estado sólido de materiales lignocelulósicos. PhD thesis dissertation. Fac. Ingenieria Quimica. Institute Superior Politécnico José A. Echevarria., Ciudad de la Habana, Cuba.
Guerra, G. (1998). Fermentación de la paja de caña de azúcar porTrichoderma citrinoviride C-9 con empleo de zeolita natural en lechos solidos. PhD thesis dissertation. Fac. de Biologia, Universidad de la Habana, Ciudad de la Habana, Cuba.
Guerra, G.Casado G.,Arguelles J.,Acebal C,Castillon P.,Torrija E.Diez C,Gómezy B.,Sñnchez MI.,León L.and Ramos-Leal M. (2003). Zeolite as source of saline nutrients in solid state fermentation of sugar cane straw byTrichoderma citrinoviride.Sugar Tech,5 (4): 243–248.
Ismail A.M,Abdel, M.and Abdel, A. (1995).Utilization of water hyacinth cellulose for production of cellobiase-rich preparation byAspergillus niger.Microbiol.,83 (336): 191–198.
León T., Arguelles J., Sánchez N., Guerra G., Casado G., Gómez and B. Sánchez M.I. (2001).Optimización del medio de cultivo para la obtención de celulasas deAspergillus niger a partir de bagazo de caña de azúcar. Rev. ICIDCA36, No1.
Lowry O.H.,Rosebroug N.J.,Farr A.L.and Randall R.J. (1951). Protein measurement with folin phenol reagent.J.Biol.Chem.,193: 265–275.
Mandels M.,Andreotti, R.and Roche, C. (1975). Measurements of saccharifying cellulose.Biotechnol. Bioeng.,6: 1471–1493.
Margolles, E.,Hayes, C,Harman, G.and Pentiila, M. (1996). Improved production ofT. harzianum endochitinase by expression inT. reeSei.Appl.Environ.Microbiol.,62: 6, p.2145–2151.
Mitchell, D. (1992). Solid substrate cultivation. Cap.6, p.87–144. Ed. by H.W.Doelle. Elselvier Applied Science, N.Y.
Ramos, L. (2000).Aplicación de la modelación matemática al desarrollo de la tecnologia del bagarip. PhD Thesis dissertation. Universidad de Camaguey, Cuba.
Sadler, J.,Yu, E.,Hartree, M.,Levitin M.and Brownell, N. (1983). Utilization of enzymatically hydrolyzed wood hemicellulase by microorganism for production of liquid fuels.Appl. Envirom. Microbiol.,45(1): 153–160.
Sasaki T.,Tanak T.,Nambu N.,Sato, Y.and Kaimura, K. (1979). Correlation between X-ray diffraction measurements of cellulose crystalline structure aand the susceptibility to microbial cellulase.Biotech. and Bioeng.,21: 1031–1042.
Sigarroa, A. (1985). Biometria y diseño experimental. 734 p. Edit. Pueblo y Educación. La Habana, Cuba.
Van Soest P.and Wine, R. (1967). Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents. Journal of A.O.A.C.20 (1): 50–55.
Winton, A.and Winton, K.B. (1944). Determinación de proteinas por el método de Barnstein. En Analisis de los Alimentas. 84 p. Edit. Ciencia y Técnica, Institute del libro, La Habana, Cuba.
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Guerra, G., Casado, M.RL.G., Arguelles, J. et al. Cellulase production with sugarcane straw byTrichoderma citrinoviride on solid bed. Sugar Tech 8, 30–35 (2006). https://doi.org/10.1007/BF02943738
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DOI: https://doi.org/10.1007/BF02943738