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Effect of Steam Explosion Pretreatment Catalysed by Organic Acid and Alkali on Chemical and Structural Properties and Enzymatic Hydrolysis of Sugarcane Bagasse

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Abstract

This study researched the steam explosion effect catalysed by citric acid and sodium hydroxide on the chemical and structural properties of sugarcane bagasse and on the enzymatic hydrolysis process. Chemical and structural characterisation of raw and pretreated biomass was performed by reference methodologies for lignocellulosic materials, FTIR, XRD, TGA and SEM. Enzymatic hydrolysis was performed with a final volume of 20 mL consisting 3% sugarcane bagasse (dry weight), sodium citrate buffer 50 mM (pH = 5.0) and 1 g enzyme/100 g cellulose of Cellic® Cetec 3 enzyme complex. The total reducing sugars were determined by a 3,5-dinitrosalicilic acid method. In explosion pretreatment catalysed by citric acid, a biomass was obtained with a lesser amount of hemicelluloses (16.16%), a higher initial degradation temperature and formation of cracks in fibre cell wall. Pretreated bagasse by NaOH steam explosion showed complete destructuring of fibre, lignin removal of 65% and hemicellulosic fraction preservation. Sugarcane bagasse obtained after NaOH steam explosion showed the highest production of reducing sugars (9.07 g L−1), which can be attributed to greater exposure of carbohydrate fraction promoted by lignin removal, since these parameters showed a strong negative correlation (r = −0.99, p < 0.05).

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Acknowledgements

The authors acknowledge the National Council for Scientific and Technological Development (CNPq); the Foundation of Support Research of the State of Minas Gerais (FAPEMIG); the Coordination for the Improvement of Higher Education Personnel (CAPES) for financial support and the Multiuser Laboratory of Microscopy of Chemical Engineering Faculty at the Federal University of Uberlândia.

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  1. Chemistry Institute, Federal University of Uberlândia, Av. João Naves de Ávila, 2121, Santa Mônica, Polymer Recycling Laboratory, Bloco 3O, Uberlândia, MG, CEP 38400-902, Brazil

    Thiago Alves Lopes Silva, Hernán Dário Zamora Zamora, Leandro Henrique Ribeiro Varão, Natália Soares Prado & Daniel Pasquini

  2. Agricultural Sciences Institute, Federal University of Uberlândia, Av. Amazonas, s/n, Umuarama, Environmental Microbiology Laboratory, Bloco 2E, Uberlândia, MG, CEP 38405-320, Brazil

    Milla Alves Baffi

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  1. Thiago Alves Lopes Silva
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Silva, T.A.L., Zamora, H.D.Z., Varão, L.H.R. et al. Effect of Steam Explosion Pretreatment Catalysed by Organic Acid and Alkali on Chemical and Structural Properties and Enzymatic Hydrolysis of Sugarcane Bagasse. Waste Biomass Valor 9, 2191–2201 (2018). https://doi.org/10.1007/s12649-017-9989-7

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