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Production of xylanase, α-l-arabinofuranosidase, β-xylosidase, and β-glucosidase by Aspergillus awamori using the liquid stream from hot-compressed water treatment of sugarcane bagasse

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Abstract

Aiming to decrease the cost of enzymes used to hydrolyze biomass, the liquid stream from the hot-compressed water (HCW) treatment of sugarcane bagasse was used in the growth medium of Aspergillus awamori 2B.361 U2/1. Statistical experimental design was used to determine the HCW1 temperature and residence time for maximum hemicellulose-derived sugar recovery to the liquid stream. The HCW reactor was fed with 10 % (w/v) bagasse, and the treatment was performed under agitation using an initial pressure of 20 bar (N2) at 170 °C for 40 min. The HCW liquid stream (23.70 ± 0.30 g/L xylan) was diluted and used as the culture medium carbon source for fungal enzyme production. In growth medium containing 5 g/L xylan at initial pH 5.0, the fungus produced 46 U/mL of xylanase (EC 3.2.1.8), 3.3 U/mL of β-glucosidase (EC 3.2.1.21), 0.24 U/mL of β-xylosidase (EC 3.2.1.37), and 0.6 U/mL of α-l-arabinofuranosidase (EC 3.2.1.55) in the supernatant. The use of a residual hemicellulosic stream would decrease the cost of enzyme production and by extension favors the economics of ethanol production from lignocellulosic biomass.

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Acknowledgments

The Research and Projects Financing (FINEP) of the Brazilian Ministry of Science and Technology, the Brazilian Research Council (CNPq), and the Japan International Cooperation Agency (JICA) supported this work. Complexo Energético Itarumã (sugar mill) is gratefully acknowledged for supplying the sugarcane bagasse for this study. The author is thankful to Ms. Reiko Yoshii and Ms. Miyu Sumii from the Biomass Refinery Research Center at the National Institute of Advanced Industrial Science and Technology (BRRC-AIST) in Japan and Ricardo S. S. Teixeira from the Enzyme Technology Laboratory (ENZITEC-UFRJ) in Brazil for the kindness and help provided during the composition of this paper.

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Authors and Affiliations

  1. Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149 Centro de Tecnologia, 21941-909, Rio de Janeiro, RJ, Brazil

    Raquel de Sousa Paredes, Rodrigo da Rocha Olivieri de Barros & Elba Pinto da Silva Bon

  2. Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (BRRC, AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan

    Hiroyuki Inoue & Shinichi Yano

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  1. Raquel de Sousa Paredes
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  2. Rodrigo da Rocha Olivieri de Barros
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  5. Elba Pinto da Silva Bon
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Correspondence to Elba Pinto da Silva Bon.

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de Sousa Paredes, R., da Rocha Olivieri de Barros, R., Inoue, H. et al. Production of xylanase, α-l-arabinofuranosidase, β-xylosidase, and β-glucosidase by Aspergillus awamori using the liquid stream from hot-compressed water treatment of sugarcane bagasse. Biomass Conv. Bioref. 5, 299–307 (2015). https://doi.org/10.1007/s13399-015-0159-5

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