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Differential expression of vitellogenin mRNA and protein in response to rice resistance genes in two strains of Nilaparvata lugens (Hemiptera: Delphacidae) with different levels of virulence

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Abstract

Controlling the brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a difficult task in rice (Oryza sativa L.) production. We focused on vitellogenins (Vg), which are the major yolk protein precursors of vitellins and play an important role in the reproduction of oviparous species, including insects. We studied the accumulation of Vg mRNA and protein in a virulent BPH strain, Nagasaki-03, and a nonvirulent strain, Hatano-66, after rearing them on four rice lines. The rice lines used were two single resistance gene introgression lines, Norin-PL3 (Bph1 carrier) and Norin-PL4 (bph2 carrier), a pyramided line in which both genes were combined, and a susceptible japonica recurrent parent Tsukushibare. RT-PCR and quantitative RT-PCR analyses showed that the Vg mRNA level decreased greatly in Hatano-66 on the resistant lines. In contrast, the level of reduction on the resistant lines was much less in Nagasaki-03. Immunoblot analysis showed that Nagasaki-03 retained comparable levels of 175 kDa Vg protein on both the susceptible and resistant lines, whereas in Hatano-66, no Vg protein was detected on the resistant lines. Our results showed that BPH resistance genes caused differential reduction in the accumulation of Vg mRNA and protein, leading to the retardation of BPH reproduction on the resistant host rice plants.

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Acknowledgments

This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology, Japan. We thank Dr. Roger F. Hou for his generous gift of chicken anti-175-kD NlVg primary antibody.

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Authors and Affiliations

  1. Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, 657-8501, Japan

    Nirosha Samanmali Gamalath, Naoki Mori & Chiharu Nakamura

  2. Laboratory of Insect Science, Graduate School of Agricultural Science, Kobe University, Kobe, 657-8501, Japan

    Muhammad Tufail & Makio Takeda

  3. Nepal Agricultural Research Council, Maize Research Program, Rampur, Chitwan, Nepal

    Prem Nidhi Sharma

Authors
  1. Nirosha Samanmali Gamalath
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  2. Muhammad Tufail
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  3. Prem Nidhi Sharma
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  4. Naoki Mori
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  5. Makio Takeda
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  6. Chiharu Nakamura
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Correspondence to Chiharu Nakamura.

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Gamalath, N.S., Tufail, M., Sharma, P.N. et al. Differential expression of vitellogenin mRNA and protein in response to rice resistance genes in two strains of Nilaparvata lugens (Hemiptera: Delphacidae) with different levels of virulence. Appl Entomol Zool 47, 9–16 (2012). https://doi.org/10.1007/s13355-011-0082-6

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