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Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India

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Abstract

A begomovirus isolate (OY136A) collected from okra plants showing upward leaf curling, vein clearing, vein thickening and yellowing symptoms from Bangalore rural district, Karnataka, India was characterized. The sequence comparisons revealed that, this virus isolate share highest nucleotide identity with isolates of Cotton leaf curl Bangalore virus (CLCuBV) (AY705380) (92.8 %) and Okra enation leaf curl virus (81.1–86.2 %). This is well supported by phylogentic analysis showing, close clustering of the virus isolate with CLCuBV. With this data, based on the current taxonomic criteria for the genus Begomovirus, the present virus isolate is classified as a new strain of CLCuBV, for which CLCuBV-[India: Bangalore: okra: 2006] additional descriptor is proposed. The betasatellite (KC608158) associated with the virus is having more than 95 % sequence similarity with the cotton leaf curl betasatellites (CLCuB) available in the GenBank.The recombination analysis suggested, emergence of this new strain of okra infecting begomovirus might have been from the exchange of genetic material between BYVMV and CLCuMuV. The virus was successfully transmitted by whitefly and grafting. The host range of the virus was shown to be very narrow and limited to two species in the family Malvaceae, okra (Abelmoschus esculentus) and hollyhock (Althaea rosea), and four in the family Solanaceae.

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Acknowledgments

The research was supported by ICAR NETWORK Project on development of diagnostics to emerging plant viruses, Indian Council of Agricultural Research, Government of India, New Delhi.

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Author notes

  1. A. Devaraju

    Present address: Phytopathology, Nunhems Pvt. Ltd., Bangalore, India

Authors and Affiliations

  1. Division of Plant Pathology, Plant Virology Laboratory, Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore, 560089, Karnataka, India

    V. Venkataravanappa, A. Devaraju, Salil Jalali & M. Krishna Reddy

  2. Indian Institute of Vegetable Research, Varanasi, 221305, Uttar Pradesh, India

    V. Venkataravanappa

  3. Department of Plant Pathology, Agriculture College, GKVK Campus, University of Agricultural Sciences (B), Bangalore, 560065, Karnataka, India

    V. Venkataravanappa

  4. Department of Plant Pathology, College of Sericulture, University of Agricultural Sciences (B), Chintamani, 563125, Karnataka, India

    C. N. Lakshminarayana Reddy

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  1. V. Venkataravanappa
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Venkataravanappa, V., Lakshminarayana Reddy, C.N., Devaraju, A. et al. Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India. Indian J. Virol. 24, 188–198 (2013). https://doi.org/10.1007/s13337-013-0141-4

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