Abstract
The complete nucleotide sequence of Abutilon mosaic Brazil virus (AbMBV), a new bipartite begomovirus from Bahia, Brazil, is described and analyzed phylogenetically. Its DNA A is most closely related to those of Sida-infecting begomoviruses from Brazil and forms a phylogenetic cluster with pepper- and Euphorbia-infecting begomoviruses from Central America. The DNA B component forms a cluster with different Sida- and okra-infecting begomoviruses from Brazil. Both components are distinct from those of the classical Abutilon mosaic virus originating from the West Indies. AbMBV is transmissible to Nicotiana benthamiana and Malva parviflora by biolistics of rolling-circle amplification products and induces characteristic mosaic and vein-clearing symptoms in M. parviflora.
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References
Bird J, Maramorosch K (1975) Tropical diseases of legumes. Academic Press, New York
Böttcher B, Unseld S, Ceulemans H, Russell RB, Jeske H (2004) Geminate structures of African cassava mosaic virus. J Virol 78:6758–6765
Castillo-Urquiza GP, Beserra JE Jr, Bruckner FP, Lima AT, Varsani A, Alfenas-Zerbini P, Zerbini MF (2008) Six novel begomoviruses infecting tomato and associated weeds in Southeastern Brazil. Arch Virol 153:1985–1989
Costa AS (1976) Whitefly transmitted plant diseases. Annu Rev Phytopathol 16:429–449
Faria JC, Maxwell DP (1999) Variability in geminivirus isolates associated with Phaseolus spp. in Brazil. Phytopathology 89:262–268
Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821
Fernandes FR, de Albuquerque LC, de Britto Giordano L, Boiteux LS, de Avila AC, Inoue-Nagata AK (2008) Diversity and prevalence of Brazilian bipartite begomovirus species associated to tomatoes. Virus Genes 36:251–258
Haible D, Kober S, Jeske H (2006) Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses. J Virol Methods 135:9–16
Inoue-Nagata AK, Albuquerque LC, Rocha WB, Nagata T (2004) A simple method for cloning the complete begomovirus genome using the bacteriophage phi29 DNA polymerase. J Virol Methods 116:209–211
Jeske H (2000) Abutilon mosaic virus. AAB Descr Plant Viruses No. 373
Jeske H (2009) Geminiviruses. In: zur Hausen H, de Villiers E-M (eds) Torque Teno Virus: The Still Elusive Human Pathogens. Springer, Berlin, pp 185–226
Jeske H, Gotthardt D, Kober S (2009) In planta cloning of geminiviral DNA: The true Sida micrantha mosaic virus. J Virol Methods 163:301–308
Jovel J, Reski G, Rothenstein D, Ringel M, Frischmuth T, Jeske H (2004) Sida micrantha mosaic is associated with a complex infection of begomoviruses different from Abutilon mosaic virus. Arch Virol 149:829–841
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) ClustalW and ClustalX version 2.0. Bioinformatics 23:2947–2948
Martin DP (2009) Recombination detection and analysis using RDP3. Methods Mol Biol 537:185–205
Morales FJ, Anderson PK (2001) The emergence and dissemination of whitefly-transmitted geminiviruses in Latin America. Arch Virol 146:415–441
Morales FJ, Jones PG (2004) The ecology and epidemiology of whitefly-transmitted viruses in Latin America. Virus Res 100:57–65
Morales FJ (2006) History and current distribution of begomoviruses in Latin America. Adv Virus Res 67:127–162
Ndunguru J, Taylor NJ, Yadav J, Aly H, Legg JP, Aveling T, Thompson G, Fauquet CM (2005) Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues. Virol J 2:45
Owor BE, Shepherd DN, Taylor NJ, Edema R, Monjane AL, Thomson JA, Martin DP, Varsani A (2007) Successful application of FTA Classic Card technology and use of bacteriophage phi29 DNA polymerase for large-scale field sampling and cloning of complete maize streak virus genomes. J Virol Methods 140:100–105
Ribeiro SG, Ambrozevicius LP, Avila AC, Bezerra IC, Calegario RF, Fernandes JJ, Lima MF, de Mello RN, Rocha H, Zerbini FM (2003) Distribution and genetic diversity of tomato-infecting begomoviruses in Brazil. Arch Virol 148:281–295
Wege C, Gotthardt RD, Frischmuth T, Jeske H (2000) Fulfilling Koch’s postulates for Abutilon mosaic virus. Arch Virol 145:2217–2225
Zhang W, Olson NH, Baker TS, Faulkner L, Agbandje-McKenna M, Boulton M, Davies JW, McKenna R (2001) Structure of the maize streak virus geminate particle. Virology 279:471–477
Acknowledgments
This work was supported by the German Academic Exchange Service (DAAD) with a scholarship to T.P. (D/06/49587) and a trilateral ERA-PG/BMBF project (to H.J., BMBF 0313986). We would like to thank Krista Frankenberry for carefully reading the manuscript, and the gardeners D. Gotthardt and A. Allinger for taking care of the plants.
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Paprotka, T., Metzler, V. & Jeske, H. The complete nucleotide sequence of a new bipartite begomovirus from Brazil infecting Abutilon. Arch Virol 155, 813–816 (2010). https://doi.org/10.1007/s00705-010-0647-7
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DOI: https://doi.org/10.1007/s00705-010-0647-7