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Introgression of QTLs determining sorghum downy mildew (SDM) resistance into elite maize line UMI 79 through marker-assisted backcross breeding (MABC)

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Abstract

Sorghum downy mildew (SDM), caused by Peronosclerospora sorghi is one of the important diseases in maize. Here, we illustrate the transfer of two QTLs for SDM on chromosome 3 and 6 from UMI 936 (w) into an elite maize inbred line UMI 79 through marker-assisted backcross breeding (MABC). For foreground selection, plants carrying heterozygote allele for the QTLs for SDM were selected from F1 and three back cross (BC1-BC3) generations using simple sequence repeat (SSR) markers linked to the targeted QTLs region of SDM. Consequently, foreground positive plants from BC3F1 generation selfed to produce BC3F2 and plants carrying the favorable alleles for the QTLs for SDM were selected followed by stringent phenotyping against SDM and advanced to BC3F3. As a result, two improved lines (79/936–1–7-7-7-46-46 and 79/936–1–7-7-10-80-80) possessing QTLs for SDM were obtained. Background selection using 146 SSR markers distributed evenly across the maize genome revealed 92.45% and 89.68% recovery of recurrent parent genome (RPG) in two improved lines. Phenotypic characterization of the improved lines showed more than 90% of recovery of the recurrent parents in morphological traits and a resistance to SDM. In conclusion, the newly developed SDM resistant inbred lines are a potential source for improving SDM resistance in maize breeding programs.

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Acknowledgements

This work was financially supported by University Grants Commission (UGC), New Delhi (F.No.40-288/2011(SR) dt.29.6.2011) and Department of Biotechnology (DBT), Government of India (GOI), under the project entitled “Molecular tagging of downy mildew resistance in maize and introgression in to the elite inbred lines” (BT/PR108910/GBD/27/111/2008 dt. 02.06.2008).

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Authors and Affiliations

  1. Department of Millets, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    K. Sumathi, P. Aarthi, V. Sruthymenon & S. Devasree

  2. Department of Genetics and Plant Breeding, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    K. N. Ganesan

  3. Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu, India

    A. Karthikeyan

  4. Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    V. Paranidharan

  5. Department of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    N. Senthil

Authors
  1. K. Sumathi
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  2. K. N. Ganesan
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  3. P. Aarthi
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  4. V. Sruthymenon
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  5. S. Devasree
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  6. A. Karthikeyan
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  7. V. Paranidharan
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  8. N. Senthil
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Contributions

NS and NKG conceived and designed the methods and experiments. KS, PA, VS and NKG constructed backcross progenies and managed the fieldwork. KS, PA, VS and MD conducted phenotype and genotype analyses. VP provided suggestions on experiments. AK, SD, KS and NS drafted the manuscript.

Corresponding author

Correspondence to N. Senthil.

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All authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Sumathi, K., Ganesan, K.N., Aarthi, P. et al. Introgression of QTLs determining sorghum downy mildew (SDM) resistance into elite maize line UMI 79 through marker-assisted backcross breeding (MABC). Australasian Plant Pathol. 49, 159–165 (2020). https://doi.org/10.1007/s13313-020-00686-9

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  • DOI: https://doi.org/10.1007/s13313-020-00686-9

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