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Expression and clinical implication of S100A12 in gastric carcinoma

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Tumor Biology

Abstract

S100 protein family has been implicated in multiple stages of tumorigenesis and progression in which S100A12 is one of the subtypes. However, the role of S100A12 in gastric carcinoma (GC) has not been elucidated yet. This study was aimed to investigate the expression of S100A12 in GC tissues and evaluate the clinical significance of S100A12 in GC patients. S100A12 protein was detected in 207 GC and 52 paired non-cancerous mucosal tissues by immunohistochemistry, while messenger RNA (mRNA) was investigated by Oncomine database analysis. Moreover, survival analysis was performed and the correlation between S100A12 and ubiquitin-specific protease 10 (USP10) and p53 was determined. As for tumor cells, the expression of S100A12 protein and mRNA in GC was proved to be lower than that in non-cancerous mucosa tissues (p < 0.05). Clinicopathological analysis showed that S100A12 protein was negatively associated with tumor size (p = 0.004), depth of invasion (p = 0.022), tumor node metastasis (TNM) stage (p = 0.018), Lauren classification (p < 0.000), and cell differentiation (p < 0.000). In contrast, a positive correlation was found between S100A12 and USP10 protein (p < 0.000). However, no relationship was detected between S100A12 and p53. Moreover, the survival analysis indicated that S100A12 protein was a favorable factor of prognosis of GC (p < 0.05). Although the expression of S100A12 in the stromal cells was detected higher than that in the tumor cells, no relationship between S100A12 protein in stromal cells and the clinicopathological features described above was found (p > 0.05). Our findings suggested that low expression of S100A12 might be served as a new marker in the tumorigenesis and progression of GC.

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Acknowledgments

This work was supported by grants from independent scientific research subject for young teachers of Wu Han University (Grant No. 2042014kf0131) and (Grant No. 2042015kf0076).

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    Authors and Affiliations

    1. Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China

      Dan Li, Jie Wu & Jin-Chun Song

    2. Department of Pathology, Renmin Hospital of Wuhan University, No 238, Jiefang Road, Wuchang District, Wuhan, 430060, Hubei, China

      Zhi Zeng, Zi-Ying Zhou & Jing-Ping Yuan

    3. Integrated Traditional Chinese and Western Medicine Ward of Oncology Department, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Techonology, Wuhan, 430014, Hubei, China

      Tao Yu

    4. Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China

      Jian Qin

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    1. Dan Li
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    Correspondence to Zhi Zeng.

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    Dan Li and Zhi Zeng contributed equally to this work.

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    Li, D., Zeng, Z., Yu, T. et al. Expression and clinical implication of S100A12 in gastric carcinoma. Tumor Biol. 37, 6551–6559 (2016). https://doi.org/10.1007/s13277-015-4460-5

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