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Role of Lgr5-positive cells in colorectal cancer

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Tumor Biology

Abstract

The molecular regulation of the growth of colorectal cancer (CRC) cells is not completely understood. Here, we report expression of Lgr5, a stem cell marker for the intestine and hair follicle, in some of the CRC cells in the patients. To determine the role of Lgr5-positive cells in the tumorigenesis of CRCs, we prepared an adeno-associated virus (AAV) that carries diphtheria toxin fragment A (DTA) under the control of Lgr5 promoter (AAV-pLgr5-DTA). Transduction of several CRC cell lines with this virus selectively killed Lgr5-positive cells, resulting in significant inhibition of the CRC cell growth in vitro and in vivo. Thus, our data highlight a potential role of Lgr5-positive cells in the tumorigenesis of CRCs, and suggest that treating these Lgr5-positive cells in CRCs may substantially improve the outcome of CRC therapy.

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Authors and Affiliations

  1. Department of Oncology, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200080, China

    Honghua Ding

  2. Department of Radiation Oncology, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China

    Chungang Wang

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  1. Honghua Ding
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  2. Chungang Wang
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Correspondence to Chungang Wang.

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Ding, H., Wang, C. Role of Lgr5-positive cells in colorectal cancer. Tumor Biol. 36, 6759–6764 (2015). https://doi.org/10.1007/s13277-015-3357-7

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  • DOI: https://doi.org/10.1007/s13277-015-3357-7

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