Effect of cancer-associated fibroblasts on the migration of glioma cells in vitro
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Cancer-associated fibroblasts (CAFs) significantly influence biological properties of many tumors. The role of these mesenchymal cells is also anticipated in human gliomas. To evaluate the putative role of CAFs in glioblastoma, we tested the effect of CAF conditioned media on the proliferation and chemotaxis of glioma cells. The proliferation of glioma cells was stimulated to similar extent by both the normal fibroblasts (NFs) and CAF-conditioned media. Nevertheless, CAF-conditioned media enhanced the chemotactic migration of glioma cells significantly more potently than the media from normal fibroblasts. In order to determine whether CAF-like cells are present in human glioblastomas, immunofluorescence staining was performed on tissue samples from 20 patients using markers typical for CAFs. This analysis revealed regular presence of mesenchymal cells expressing characteristic CAF markers α-smooth muscle actin and TE-7 in human glioblastomas. These observations indicate the potential role of CAF-like cells in glioblastoma biology.
KeywordsCancer-associated fibroblasts Glioma Mesenchymal cells Tumor microenvironment
This work was supported by the following grants: Grant Agency of the Charles University in Prague (GAUK) project 44214, Internal Grant Agency of the Ministry of Health of the Czech Republic (IGA) project 12237-5/2011, University Research Centers (UNCE) project 204013, Charles University Research Development Schemes (PRVOUK) project P27/LF1/1, Czech Science Foundation (GAČR) project P304-12-1333, Specific Academic Research Projects (SVV) project 260032, and by the project “BIOCEV – Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University” (CZ.1.05/1.1.00/02.0109), from the European Regional Development Fund.
An informed consent was obtained from all individual participants included in the study. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.
Conflicts of interest
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