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Assessment of genetic diversity and population structure among a collection of Korean Perilla germplasms based on SSR markers

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Abstract

Background

Information on the genetic variation of genetic resource collections is very important for both the conservation and utilization of crop germplasms in genebanks. Var. frutescens of Perilla crop is extensively cultivated in South Korea as both an oil crop and a vegetable crop.

Objectives

We used SSR markers to evaluate the genetic diversity, genetic relationships, and population structure of 155 accessions of var. frutescens that have been selected as genetic resources for the development of leaf vegetable cultivars and preserved in the RDA-Genebank collection from South Korea.

Methods

A total of 155 accessions of var. frutescens of Perilla crop collected in South Korea were obtained from the RDA-Genebank of the Republic of Korea. We selected 20 SSR markers representing the polymorphism of and adequately amplifying all the Perilla accessions.

Results

The average GD and PIC values were 0.642 and 0.592, respectively, with ranges of 0.244–0.935 and 0.232– 0.931. The genetic variability in the southern region of South Korea was higher than that in the central region. The clustering patterns were not clearly distinguished between the accessions of var. frutescens from the central and southern regions of South Korea.

Conclusion

These results regarding the genetic diversity and population structure of the 155 accessions of var. frutescens of South Korea provide useful information for understanding the genetic variability of this crop and selecting and managing core germplasm sets in the RDA-Genebank of the Republic of Korea.

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Acknowledgments

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (#2016R1D1A1B01006461), and the Cooperative Research Program for Agriculture Science and Technology Development (project nos. PJ014227032019 and PJ0142272019), Rural Development Administration, Republic of Korea.

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Author notes

  1. Jun Seok Oh, Kyu Jin Sa have contributed equally to this work.

Authors and Affiliations

  1. Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, 24341, South Korea

    Jun Seok Oh, Kyu Jin Sa & Ju Kyong Lee

  2. National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju, 54874, South Korea

    Do Yoon Hyun & Gyu-Taek Cho

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  1. Jun Seok Oh
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Correspondence to Ju Kyong Lee.

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Oh, J.S., Sa, K.J., Hyun, D.Y. et al. Assessment of genetic diversity and population structure among a collection of Korean Perilla germplasms based on SSR markers. Genes Genom 42, 1419–1430 (2020). https://doi.org/10.1007/s13258-020-01013-8

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