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Identification and expression patterns of 20-hydroxyecdysone-responsive genes from Procambarus clarkii

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Abstract

Ecdysteroids play an important role in the regulation of molting process in crustaceans. The suppression subtractive hybridization (SSH) technique was employed to search for 20-hydroxyecdysone-responsive genes in the hepatopancreas of the crayfish Procambarus clarkii. Ninety-three putative expressed sequence tags were identified by SSH, including three immune response-related genes, two cell cycle and apoptosis genes, four respiration and energy metabolism genes, four transport-related genes, six metabolism-related genes, two stress response genes, and eight transcription and translation regulation genes. The expression levels of the examined genes were regulated by 20-hydroxyecdysone and varied in the hepatopancreas during the molting stages; data were confirmed by real-time PCR. RNAi of the ecdysteroid receptor had significant effects on the expression levels of 20-hydroxyecdysone-responsive genes. These differentially expressed genes identified by SSH will provide insight into the ecdysteroid signaling pathway in P. clarkii.

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Acknowledgements

This work was supported by Natural Science Foundations of Anhui Province of China (1508085MC52), Research Fund for the Doctoral Program of Higher Education of China (20123418120003), Training Project for Backbone Teacher (2014XKPY-38), Natural Science Foundation of the Anhui Higher Education Institutions of China (KJ2014A071).

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  1. College of Life Sciences, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, People’s Republic of China

    Bao-Jian Zhu, Lin Tang, Ying-Ying Yu, Dao-Jun Wang & Chao-Liang Liu

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  1. Bao-Jian Zhu
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  2. Lin Tang
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Correspondence to Bao-Jian Zhu.

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Zhu, BJ., Tang, L., Yu, YY. et al. Identification and expression patterns of 20-hydroxyecdysone-responsive genes from Procambarus clarkii . Genes Genom 39, 601–609 (2017). https://doi.org/10.1007/s13258-017-0527-6

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  • DOI: https://doi.org/10.1007/s13258-017-0527-6

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