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Transcriptome and digital gene expression analysis of herbaceous peony (Paeonia lactiflora Pall.) to screen thermo-tolerant related differently expressed genes

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Abstract

Herbaceous peony (Paeonia lactiflora Pall.) is easily injured by heat stress (HS), which greatly restricts its application and promotion. In this study, the thermo-tolerance of three representative P. lactiflora cultivars had been firstly assessed. ‘Zifengyu’ was identified as the thermo-tolerant cultivar with relatively lower values and smaller variations in malondialdehyde, hydrogen peroxide (H2O2) and proline contents under HS. Subsequently, their transcriptomes were sequenced by RNA sequencing (RNA-seq) technology to construct a complete database. 81,599 unigenes were obtained, and 34,940 unigenes had been annotated. Moreover, through digital gene expression analysis of thermo-tolerant ‘Zifengyu’ and moderately thermo-tolerant ‘Hongyanzhenghui’, 161 heat stress response genes had been screened involving heat shock protein genes, plant hormone signal transduction related genes, fatty acid synthesis genes, reactive oxygen species-scavenging genes and secondary metabolites related genes. And the effectively and timely response of these genes to HS could endow thermo-tolerance to ‘Zifengyu’. Among these genes, 11 key thermo-tolerant related genes whose expressions were all significantly up-regulated in ‘Zifengyu’ and ‘Hongyanzhenghui’ during development and the former possessed higher levels could be regarded as the candidate genes, including isoprene synthase gene, 2 peroxidase genes, 3-oxoacyl-acyl carrier protein reductase gene (FabG), 3 transcription factor genes (bHLH, NAC and WRKY), HSP20 and 3 HSP70. These results could provide a better understanding of heat stress response in P. lactiflora, and pave for the breeding of thermo-tolerant cultivars.

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Acknowledgments

This work was supported by the College Natural Science Research of Jiangsu Province (14KJB210011), the Opening Project of Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement (2014014), the Natural Science Foundation of Yangzhou City (YZ2014033), and the Priority Academic Program Development from Jiangsu Government.

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Authors and Affiliations

  1. College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, People’s Republic of China

    Zhaojun Hao & Jun Tao

  2. Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, Jiangsu, People’s Republic of China

    Zhaojun Hao, Mengran Wei, Saijie Gong, Daqiu Zhao & Jun Tao

  3. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, 210014, Jiangsu, People’s Republic of China

    Daqiu Zhao & Jun Tao

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  1. Zhaojun Hao
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  2. Mengran Wei
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Correspondence to Jun Tao.

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Zhaojun Hao, Mengran Wei, Saijie Gong, Daqiu Zhao and Jun Tao declares no conflict of interest.

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Hao, Z., Wei, M., Gong, S. et al. Transcriptome and digital gene expression analysis of herbaceous peony (Paeonia lactiflora Pall.) to screen thermo-tolerant related differently expressed genes. Genes Genom 38, 1201–1215 (2016). https://doi.org/10.1007/s13258-016-0465-8

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