Skip to main content
Log in

Cytological and morphological characterization of rye-wheat derivatives for important agronomic traits

  • Original Article
  • Published:
The Nucleus Aims and scope Submit manuscript

Abstract

Ninety two derivatives derived from rye-wheat crosses were analyzed for their rye chromosome/chromatin constitution using molecular cytogenetic tools FISH and GISH, to detect targeted rye chromatin introgression with minimum linkage drag. Of the 92 recombinants lines analyzed, 17 exhibited one pair of rye chromosome substituting different chromosomes of A, B, and D genomes, 10 lines showed substitution and translocations at the same time and 55 lines had 1BL.1RS translocations. Four lines that had more than 10 chromosomes from rye were alike triticale, whereas remaining six lines exhibited no rye chromatin/chromosome. These lines were evaluated for different agronomic traits such as days to maturity, type of spike, colour of spike, compactness of spike etc. for ascertaining correlation between rye chromatin with morphological traits.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Carvalho R, Guedes-Pinto CH, Heslop-Harrison JS, Schwarzacher T. Introgression of rye chromatin on chromosome 2D in the Portuguese wheat landrace barbela. Genome. 2001;44:1122–8.

    Article  Google Scholar 

  2. Castilho A, Miller TE, Heslop-Harrison JS. Physical mapping of translocation breakpoints in a set of wheat-Aegilops umbellulata recombinant lines using in situ hybridization. Theor Appl Genet. 1996;93:816–25.

    Article  CAS  PubMed  Google Scholar 

  3. Friebe B, Gill BS. C-band polymorphism and structural rearrangements detected in common wheat (Triticum aestivum). Euphytica. 1994;78:1–5.

    Google Scholar 

  4. Friebe B, Mukai Y, Dhaliwal HS, Martin TJ, Gill BS. Identification of alien chromatin specifying resistance to wheat streak mosaic virus and green-bug in wheat germplasm by C-banding and in-situ hybridization. Theor Appl Genet. 1991;81:381–9.

    Google Scholar 

  5. Gill BS, Friebe B, Endo TR. Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome. 1988;199:830–9.

    Google Scholar 

  6. Hu Y, Bao RR, Xue XY. The new strain ‘764’ of spring wheat by pollen haploid technique from Anther culture. Genet Manip Crops Newslett. 1988;4:70–85.

    Google Scholar 

  7. Le HT, Armstrong KC, Miki B. Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Plant Mol Bio Rep. 1989;7:150–15.

    Article  Google Scholar 

  8. McIntyre CL, Pereira SL, Moran B, Appels R. New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome. 1990;33:635–40.

    Article  CAS  PubMed  Google Scholar 

  9. Mukai Y, Friebe B, Gill BS. Comparison of C-banding pattern and in situ hybridization sites using highly repetitive and total genomic rye DNA probes of ‘imperial’ rye chromosomes added to ‘Chinese spring’ wheat. Jpn J Genet. 1992;67:71–8.

    Article  Google Scholar 

  10. Mukai Y, Nakahara Y, Yamamoto M. Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome. 1993;36:489–94.

    Article  CAS  PubMed  Google Scholar 

  11. Nagy DE, Molnar-Láng M, Linc G, Láng L Identification of wheat–barley translocations by sequential GISH and two-colour FISH in combination with the use of genetically mapped barley SSR markers. Genome. 2002;45:1238–47.

    Article  CAS  PubMed  Google Scholar 

  12. Pedersen C, Langridge P. Identification of the entire chromosome complement of bread wheat by two-colour FISH. Genome. 1997;40:593–8.

    Article  Google Scholar 

  13. Rayburn AL, Gill BS. Molecular identification of the D-genome chromosomes of wheat. J Hered. 1986b;77:253–5.

    CAS  Google Scholar 

  14. Taketa S, Nakazaki T, Schwarzacher T, Heslop-Harrison JS. Detection of a 4DL chromosome segment translocated to rye chromosome 5R in an advanced hexaploid triticale line bronco 90. Euphytica. 1997;97:91–6.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to R. K. Chahota.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chahota, R.K., Mukai, Y., Sharma, T.R. et al. Cytological and morphological characterization of rye-wheat derivatives for important agronomic traits. Nucleus 58, 211–216 (2015). https://doi.org/10.1007/s13237-016-0158-3

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13237-016-0158-3

Keywords

Navigation