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Molecular cytogenetic analysis of newly developed progenies from triticale × wheat crosses for yield and stress tolerance

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Abstract

A total of 139 triticale × wheat progenies (including recombinant inbred lines and doubled haploids) were developed indigenously. Screening at cytogenetic level using genomic in situ hybridization and fluorescence in situ hybridization revealed maximum progenies (48%) carrying 1RS.1BL translocation. D-R genome substitution ranging from 1 to 7 chromosomes of rye showed significantly more biological yield (9.29 g), 1000 grain weight (48.26 g), number of tillers per plant (6.67) and plant height (106.06 cm) than other progenies depicting the contribution of rye chromatin towards plant yield. Thirteen per cent of lines were found without rye chromatin. Screening of all progenies against yellow rust pathogen revealed about 85% lines to be resistant with varying levels of resistance. In vitro testing for drought tolerance showed non-significant difference among progenies carrying variable rye introgressions. The progenies developed in the present study may present novel germplasm and utilized in the future wheat improvement programmes.

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Acknowledgements

The authors are highly thankful to Monsanto’s for providing financial support through prestigious Beachell Borlaug International Fellowship-2012.

Funding

The authors are thankful to Monsanto’s Beachell Borlaug International Fellowship-2012 for providing financial support for executing the present research work.

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Authors and Affiliations

  1. PAU, Regional Research Station, Kapurthala, 144601, Punjab, India

    N. S. Jamwal

  2. Molecular Cytogenetic and Tissue Culture Lab, CSKHPAU, Himachal Pradesh, Palampur, 176062, India

    A. Badiyal & H. K. Chaudhary

  3. Molecular Biology Lab, University of Leicester, Leicester, UK

    Trude Schwarzacher & J. S. Heslop-Harrison

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  1. N. S. Jamwal
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  2. A. Badiyal
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  3. H. K. Chaudhary
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  4. Trude Schwarzacher
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  5. J. S. Heslop-Harrison
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All the authors have contributed towards planning, execution and presentation of the work.

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Correspondence to H. K. Chaudhary.

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No animals were used in the study.

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The authors declare that the work is original and complete. The work has not been submitted to any other journal for publication. The results presented in the manuscript are original.

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Communicated by Márta Molnár-Láng.

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Jamwal, N.S., Badiyal, A., Chaudhary, H.K. et al. Molecular cytogenetic analysis of newly developed progenies from triticale × wheat crosses for yield and stress tolerance. CEREAL RESEARCH COMMUNICATIONS (2023). https://doi.org/10.1007/s42976-023-00410-5

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