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Morphological property and in vitro enzymatic degradation of modified chitosan as a scaffold

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An Erraum to this article was published on 04 January 2012

Abstract

Chitosan (CS) was proposed as a promising candidate scaffold for tissue engineering. However, some drawbacks of natural CS remain. The current study modified CS by conjugating thiol to CS polymer (Thio-CS) and substantiated its three-dimensional microstructure and physical properties such as swelling or degradation. The Thio-CS was obtained by CS modification using 2-iminothiolane-HCl (2-IT). Because of the formation of disulfide bonds between thiol moieties based on oxidation of the immobilized thiol groups of CS, Thio-CS exhibits in situ gelling properties according to the reducing amount of free thiol. The content of the thiol group was increased as the amount of 2-IT increased. The swelling test demonstrated that Thio-CS can absorb up to 3.5 times its weight of phosphate buffered saline within 1 h and that the pore size and amount significantly increased with incubation time. The Thio-CS enzymatic degradation rate according to velocity was investigated. The results showed that Thio-CS was more resistant to lysozyme as viscosity increased. Thio-CS sponges were fabricated using freezedrying. The lyophilized Thio-CS had a homogeneous honeycomb-like shape, and its pores were relatively smaller (<2 μm) than those of unmodified CS (>2 μm). These results suggest that Thio-CS might be a candidate regenerative therapeutic device.

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Authors and Affiliations

  1. Dental Science Research Institute and BK21 Project, School of Dentistry, Chonnam National University, Gwangju, 500-757, Korea

    In-Ho Bae, Won Gu Jang, Hyun-Pil Lim, Sang-Won Park, Young-Joon Park & Jeong-Tae Koh

  2. Division of Materials Science and Engineering, Chonnam National University, Gwangju, 500-757, Korea

    Kwang-Min Lee

  3. Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju, 501-746, Korea

    In-Kyu Park

  4. Heart Research Center, Chonnam National University Hospital, Gwangju, 501-757, Korea

    In-Ho Bae, In-Kyu Park & Myung Ho Jeong

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  1. In-Ho Bae
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  2. Won Gu Jang
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Correspondence to Jeong-Tae Koh.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s13233-012-0193-0

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Bae, IH., Jang, W.G., Lim, HP. et al. Morphological property and in vitro enzymatic degradation of modified chitosan as a scaffold. Macromol. Res. 19, 1250–1256 (2011). https://doi.org/10.1007/s13233-011-1203-3

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