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Conjugational delivery of chromosomal integrative constructs for gene expression in the carbendazim-degrading Rhodococcus erythropolis D-1

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Abstract

Rhodococcus strains not only have been widely used in industries but also have a potential ability of producing new structural natural products. Integration of heterologous genes into chromosomes of Rhodococcus strains for gene expression can facilitate the studies and applications of these strains. A conjugation system was optimized in order to transfer enhanced green fluorescent protein (EGFP) encoding gene as a reporter from Escherichia coli into Rhodococcus erythropolis D-1. The influence of three native ribosome binding sites (RBSs) and two designed RBSs on the target protein production in R. erythropolis D-1 was also characterized. An efficient conjugation system of R. erythropolis D-1 was established to integrate EGFP gene into its chromosome. Among of five RBSs, RBS3 showed the highest translational activity in R. erythropolis D-1.

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Funding

This work was financially supported by the Zhejiang Provincial Natural Science Foundation of China (LR16H300001), the National Natural Science Foundation of China (31670008), and the State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University (MMLKF17-06).

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Author notes

  1. Yang Tao and Wen-Guang Wang contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China

    Yang Tao, Wen-Guang Wang, Sheng-Hui Lu, Xin Xie, Ugit Lazzat, Yuhua Zhao & Hui Jiang

  2. Eco-environmental Protection Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China

    Naling Bai

Authors
  1. Yang Tao
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  2. Wen-Guang Wang
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  3. Sheng-Hui Lu
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  4. Xin Xie
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  5. Ugit Lazzat
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  6. Naling Bai
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  7. Yuhua Zhao
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Correspondence to Yuhua Zhao or Hui Jiang.

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Tao, Y., Wang, WG., Lu, SH. et al. Conjugational delivery of chromosomal integrative constructs for gene expression in the carbendazim-degrading Rhodococcus erythropolis D-1. Ann Microbiol 68, 773–780 (2018). https://doi.org/10.1007/s13213-018-1382-7

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  • DOI: https://doi.org/10.1007/s13213-018-1382-7

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