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Abundance and ribotypes of phosphate-solubilizing bacteria in Argentinean agricultural soils under no-till management

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Abstract

Phosphate-solubilizing bacteria (PSB) abundance and ribotypes were examined in the top soil (0–10 cm) of agricultural fields under no-till management sampled in two seasons, summer (February) and late winter (September), in 2010. No-till plots under sustainable agricultural practices (intense crop rotation) or under non-sustainable practices (soybean monocropping) were sampled at four different locations as replicates in a 400-km west–east transect in the most productive agricultural region of Argentina. Natural grasslands were selected close to the cultivated fields for comparative purposes. Culturable heterotrophic bacteria (CHB) were enumerated on nutrient agar plates, and PSB were counted on NBRIP agar plates containing Ca3(PO4)2. The PSB community structure was explored by ribotyping (16S rDNA PCR-restriction fragment length polymorphism). Quantitatively, data showed that the number of CHB and PSB per gram of dry soil was not statistically different among sampled sites, soil management programs or seasons. Qualitatively, ribotyping showed that the most abundant PSB species differed in their fingerprinting patterns among geographical sites, which suggests that local soil conditions impose strong selective constraints. The comparative analysis of PSB ribotypes revealed seasonal differences among February and September isolates for all sampling sites.

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Acknowledgments

This work was supported by grants PAE 36976-PID 52 (Agencia Nacional de Promoción Científica y Tecnológica, Argentina), PIP 112-200801-02271 (CONICET, Argentina) and PUNQ 0395/07 (Universidad Nacional de Quilmes, Argentina). We would like to thank Marina Díaz, Liliana Gallez, and Celina Zabaloy from Universidad Nacional del Sur for providing laboratory facilities to carry out experimental ribotype analysis of PSB isolates of part of the samples. LF and BA were supported by CONICET through post-doctoral and Ph.D. fellowships, respectively. LGW and CV are members of CONICET.

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Authors and Affiliations

  1. Laboratorio de Microbiología Agrícola, Departamento de Agronomía, Universidad Nacional del Sur, San Andrés s/n Altos del Palihue, Bahía Blanca, 8000, Buenos Aires, Argentina

    Leticia A. Fernández

  2. Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal, B1876BXD, Buenos Aires, Argentina

    Betina Agaras, Luis G. Wall & Claudio Valverde

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  1. Leticia A. Fernández
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  2. Betina Agaras
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Correspondence to Leticia A. Fernández.

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Fernández, L.A., Agaras, B., Wall, L.G. et al. Abundance and ribotypes of phosphate-solubilizing bacteria in Argentinean agricultural soils under no-till management. Ann Microbiol 65, 1667–1678 (2015). https://doi.org/10.1007/s13213-014-1006-9

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