Abstract
The prized Red banana, selected for superior qualities, demands strong genetic uniformity for successful clonal propagation and preservation. Ensuring this uniformity early in the growth of in vitro Red banana plants is essential, as gene mutations and chromosome rearrangements during tissue culture can jeopardize both cloning and germplasm conservation. In this situation, molecular markers play a pivotal role in confirming genetic stability. Thus the study aims to discover a marker that identifies tissue-cultured Red bananas from their virescent variants during initial sub-culturing. A marker linked to anthocyanin has been identified which effectively differentiated Red bananas from virescent variants and it was further validated in various banana cultivars, ornamental Musa species and their interspecific hybrids. The PCR-based marker showed remarkable specificity, discerning Red bananas from virescent variants during tissue culture. It also distinguished green and red offspring, cutting time and resource costs, and shortening the banana breeding cycle.
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Availability of data and materials
The datasets generated or analyzed during the current study are available from the corresponding author on reasonable request. All other data generated or analyzed during this study are included in this article supplementary information files.
Abbreviations
- ECS:
-
Embryogenic cell suspensions
- F3′5ʹH1:
-
Flavonoid 3ʹ, 5ʹ-hydroxylase-1
- TA:
-
Total anthocyanin
- TC:
-
Tissue culture
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Acknowledgements
The financial support received from the Indian Council of Agricultural Research, New Delhi is greatly acknowledged.
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Grant ID. IXX14668, IXX12449, Indian Council of Agricultural Research, New Delhi.
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AC: Project administration, conceptualization, investigation, work design, data curation, methodology, marker development, validation of markers, visualization, analyzed the data, Writing—Original draft, review and editing, Formatting; RD: Conceptualization, data curation, Plant materials, ornamental parents and hybrids, statistical analysis; MM: anthocyanin estimation, HPLC; BS: in vitro plants materials, editing; PP: validation; MVK: validation; AS: validation; US: validation.
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Anuradha, C., Ramajayam, D., Mayilvaganan, M. et al. Molecular characterization of Red banana and its somaclonal variant: a comprehensive study. 3 Biotech 14, 19 (2024). https://doi.org/10.1007/s13205-023-03868-6
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DOI: https://doi.org/10.1007/s13205-023-03868-6