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Bottle gourd IC-0262269, a super-susceptible genotype to tomato leaf curl Palampur virus

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Abstract

While conducting field trial of 82 genotypes of bottle gourd at Delhi during 2020–2021, a particular genotype, IC-0262269 was found to be affected by chlorotic curly stunt disease (CCSD). The affected plants were severely stunted and bearing very small chlorotic and crinkle leaves. The disease incidence in the said genotype was as high as 80% among different replicated trial blocks. The application of PCR using a generic primers specific to begomoviruses, as well as species-specific PCR diagnostics to six tomato-infecting begomoviruses: tomato leaf curl New Delhi virus (ToLCNDV), tomato leaf curl Palampur virus (ToLCPalV), tomato leaf curl Joydebpur virus (ToLCJoV), tomato leaf curl Gujrat virus (ToLCGuV), tomato leaf curl Bangalore virus (ToLCBV), and chilli leaf curl virus (ChiLCV) showed that, only ToLCPalV could be detected in the genotype IC-0262269. Following, rolling circle amplification, cloning and sequencing of full-length DNA-A and DNA-B genome of an isolate BoG1-ND from the genotype IC-0262269 revealed association of ToLCPalV with the disease. The successful agro-infection of the cloned genome of BoG1-ND (DNA-A and DNA-B) in the plants of Nicotiana benthamiana and bottle gourd demonstrated that ToLCPalV is the causal begomovirus of CCSD. The study provides the first evidence of the natural occurrence of ToLCPalV in bottle gourd crop and also showed that the bottle gourd genotype IC-0262269 is super-susceptible to ToLCPalV.

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Data availability

The sequence information data of DNA-A and DNA-B genomic components of tomato leaf curl Palampur virus have been deposited in GenBank and are available under the accession numbers OQ589856 and OQ589857.

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Acknowledgements

The first author is thankful to the Ministry of Tribal Affairs, Govt. of India for providing the Ph.D. fellowship. The financial support from the project is thankfully acknowledged. The financial support from the research grant under the Consortia Research Platform on Vaccines & Diagnostics, Indian Council of Agricultural Research, New Delhi is thankfully acknowledged.

Funding

The financial support from the research grant under the Ref: F. No. 16–11/PP/ICAR-CRP/20–22/04 Consortia Research Platform on Vaccines & Diagnostics, Indian Council of Agricultural Research, New Delhi is thankfully acknowledged.

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  1. Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    S. Naveen Nayaka, Firoz Mondal, Anirban Roy & Bikash Mandal

  2. Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Jeetendra Kumar Ranjan

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  1. S. Naveen Nayaka
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Nayaka, S.N., Mondal, F., Ranjan, J.K. et al. Bottle gourd IC-0262269, a super-susceptible genotype to tomato leaf curl Palampur virus. 3 Biotech 14, 8 (2024). https://doi.org/10.1007/s13205-023-03838-y

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