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Molecular characterization of a distinct bipartite Begomovirus species infecting ivy gourd (Coccinia grandis L.) in Tamil Nadu, India

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Abstract

A distinct bipartite begomovirus was found to be associated with the mosaic disease on ivy gourd (Coccinia grandis L.) in Tamil Nadu, India. The complete DNA A and DNA B components were cloned by rolling circle amplification. Genome organization of this virus is found to be typical of Old World bipartite begomovirus. The association of betasatellite component with this virus is absent. The closest nucleotide identity of 73.4 % was seen with the Loofa yellow mosaic virus (LYMV-[VN]-AF509739) suggesting that it is a new virus species Coccinia mosaic virus (CoMoV-Ivy gourd [TN TDV Coc1]) and distantly related to the other known begomoviruses. The DNA B component shared a maximum identity of 55 % with that of Tomato leaf curl New Delhi virus (ToLCNDV). In the phylogenetic analysis, CoMoV-Ivy gourd form cluster separate from other begomoviruses. Recombination analysis showed that there was no recombination event in the genome. This is the distinct begomovirus infecting ivy gourd.

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Acknowledgments

This publication was made possible through Cooperative Agreement No. EPP-A-00-0400016-00 of USAID for the IPM Innovation Lab. The opinions expressed herein are those of the authors and do not necessarily reflect the views of the USAID. We thank Dr. R.A. Naidu (WSU, USA) for his valuable guidance and Dr. V.G. Malathi for her critical comments on the manuscript.

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Authors and Affiliations

  1. Division of Crop Protection, ICAR - Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, India

    K. Nagendran

  2. National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban, Tamil Nadu, India

    V. K. Satya

  3. Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    S. Mohankumar

  4. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    K. Nagendran & G. Karthikeyan

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  1. K. Nagendran
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Correspondence to K. Nagendran.

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Edited by Karel Petrzik.

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Nagendran, K., Satya, V.K., Mohankumar, S. et al. Molecular characterization of a distinct bipartite Begomovirus species infecting ivy gourd (Coccinia grandis L.) in Tamil Nadu, India. Virus Genes 52, 146–151 (2016). https://doi.org/10.1007/s11262-015-1278-6

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