Abstract
The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium Bacillus smithii IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (t1/2) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA.
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Funding
The authors are thankful for the financial assistance from the Science and Engineering Research Board, Department of Science and Technology, Government of India (Grant no-SB/YS/LS-15/2014 and LAB Projects (MLP 1003 MLP1008). A Fellowship grant by the Council of Scientific and Industrial Research (CSIR) to Hitesh Sharma is also acknowledged. In addition, the Indian Council of Medical Research (ICMR) is also appreciated for the fellowship grant of Ananta Ganjoo (Letter no. 5/3/8/ITR-F/2020 Dated: 28/02/2020). IIIM communication no. CSIR-IIIM/IPR/00129, Dated: 11/27/2019.
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13205_2022_3109_MOESM1_ESM.tiff
Supplementary Fig S1. Zymogram of (L1) Total cell lysate and; (L2) Partially purified amidase (Brown colour band indicating amidase of Bacillus sp. IIIMB2907) (TIFF 1118 KB)
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Sharma, H., Singh, R.V., Ganjoo, A. et al. Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907. 3 Biotech 12, 44 (2022). https://doi.org/10.1007/s13205-022-03109-2
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DOI: https://doi.org/10.1007/s13205-022-03109-2