Abstract
To explore the potential function of miR-9-5p in wear-particle-induced osteoclastogenesis, we examined the expression of SIRT1 and miR-9-5p in particle-induced osteolysis (PIO) mice calvariae and polyethylene (PE)-induced RAW 264.7 cells and found that SIRT1 expression was downregulated while miR-9-5p expression was upregulated in both models. We then verified that miR-9-5p targets SIRT1. miR-9-5p was found to promote PE-induced osteoclast formation from RAW 264.7 cells by tartrate-resistant acid phosphatase staining and detection of osteoclast markers, and miR-9-5p activation of the SIRT1/NF-kB signaling pathway was found in cells by detecting the expression of SIRT1/NF-kB pathway-related proteins and rescue assays. In conclusion, we found that miR-9-5p activated the SIRT1/NF-κB pathway to promote wear-particle-induced osteoclastogenesis. miR-9-5p may be a useful therapeutic target for PIO remission and treatment.
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This study was supported by Natural Science Foundation of Ningxia Province (Grant No. 2019AAC03209).
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Liang Zhang designed the research. Liang Zhang wrote the manuscript with contributions from all authors. All authors participated in part of the experiment. Weidong Zhao and Dongmei Bao are responsible for data acquisition and analysis. Zhihui Gao is responsible for statistical analysis. Kening Sun and Peng Li are responsible for the literature search. Zhidong Lu reviewed and modified the article. All authors read and approved the final manuscript.
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This experiment abides by the principle of experimental animals. All experimental animals are treated in accordance with the principles of experimental animals, and the experiment is approved by the Ethics Committee of the General Hospital of Ningxia Medical University. The permission number for ethical approval of animals was 2019-241.
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Zhang, L., Zhao, W., Bao, D. et al. miR-9-5p promotes wear-particle-induced osteoclastogenesis through activation of the SIRT1/NF-κB pathway. 3 Biotech 11, 258 (2021). https://doi.org/10.1007/s13205-021-02814-8
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DOI: https://doi.org/10.1007/s13205-021-02814-8