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Biodegradation and biodetoxification of batik dye wastewater by laccase from Trametes hirsuta EDN 082 immobilised on light expanded clay aggregate

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Abstract

The biodegradation and biodetoxification of batik industrial wastewater by laccase enzyme immobilised on light expanded clay aggregate (LECA) were investigated. Laccase from Trametes hirsuta EDN 082 was covalently immobilised by modifying the LECA surface using (3–aminopropyl)trimethoxysilane and glutaraldehyde. The enzymatic characterisation of LECA–laccase showed promising results with an enzyme loading of 6.67 U/g and an immobilisation yield of 66.7% at the initial laccase activity of 10 U/g LECA. LECA-laccase successfully degraded batik industrial wastewater containing indigosol dye up to 98.2%. In addition, the decolorisation extent was more than 95.4% after four cycles. The phytotoxicity assessment of Vigna radiata and the microbial toxicity of two pathogenic bacteria, Bacillus subtilis and Pseudomonas aeruginosa, showed biodetoxification of treated batik dye wastewater. The characterisation using 3D light microscopy, scanning electron microscopy and Fourier transform infrared for LECA–laccase confirmed that laccase was successfully immobilised on LECA, and the decolorisation achieved through the combination of adsorption and enzymatic degradation. This study offers an environmentally friendly, effective and affordable LECA–laccase as a method for batik dye wastewater treatment.

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Acknowledgements

Part of this research was supported by the Indonesian Institute of Sciences (LIPI) through DIPA 2020 of the Deputy for Life Sciences, by the Japan ASEAN Science, Technology and Innovation Platform (JASTIP) and by the Humanosphere Asia Research Node (ARN) program of the Research Institute for Sustainable Humanosphere, Kyoto University. The authors also acknowledge the facilities and the scientific and technical assistance of the Integrated Laboratory of Bioproducts at the LIPI.

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DHYY: Conceptualisation, supervision, funding acquisition, formal analysis, original draft. MAG: Experiment, original draft. ODN: Formal analysis. SHA: Formal analysis. MO: Formal analysis. KPR: Formal analysis. MFP: Supervision. TW: Supervision, funding acquisition, review draft.

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Correspondence to Dede Heri Yuli Yanto.

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Yanto, D.H.Y., Guntoro, M.A., Nurhayat, O.D. et al. Biodegradation and biodetoxification of batik dye wastewater by laccase from Trametes hirsuta EDN 082 immobilised on light expanded clay aggregate. 3 Biotech 11, 247 (2021). https://doi.org/10.1007/s13205-021-02806-8

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  • DOI: https://doi.org/10.1007/s13205-021-02806-8

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