Abstract
The high pigment (hp) tomato mutants increase the fruit pigment content in tomato. The dark green (dg) tomato mutant belonging to this category contains the hp-2dg recessive allele, resulting from a single nucleotide polymorphism (SNP) in the DEETIOLATED1 (DET1) gene. Naturally, identification of this functional SNP is crucial for introgression of this important mutant allele in suitable tomato cultivar(s). Hence, we developed a functional co-dominant marker for detecting the hp-2dg mutant allele using the tetra-primer amplicon refractory mutation system (T-ARMS) strategy. Although the other available techniques for identification of SNPs require specialized costly equipments and reagents, our method allows the reliable identification of this functional SNP in time-, labour- and cost-effective manner, using standard molecular biology equipments and reagents. To the best of our knowledge, this is the first report of T-ARMS strategy for the detection of the dg mutant allele in tomato, which will definitely help in identification and introgression of this allele by the breeders, working even under limited fund and resources.
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Acknowledgements
We thank Prof. Pranab Hazra, BCKV for providing seeds of Aft and dg mutant lines and “purple tomato” genotype. ICAR-IIHR, Bengaluru, ICAR-IIVR Varanasi and NBPGR are acknowledged for providing other seed materials. The authors thank Deepak Maurya, Shubham Kumar and Vikash Kumar for their help. Financial support from BAU in terms of project grant (Code: SNP/CI/Rabi/2018-5) is highly acknowledged. This article bears BAU COMMUNICATION NO. 889/200930.
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TC conceived the idea. AM and TC designed the primers and performed experiments. SA, AM and TC carried out crop production and plant hybridization works. All the authors took part in preparation and correction of the manuscript. All the authors read the final manuscript and approved it.
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Mukherjee, A., Akhtar, S. & Chattopadhyay, T. Development of tetra-primer amplicon refractory mutation system (T-ARMS) strategy for identification of the dark green mutant allele in tomato. 3 Biotech 11, 182 (2021). https://doi.org/10.1007/s13205-021-02732-9
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DOI: https://doi.org/10.1007/s13205-021-02732-9