Abstract
Tuber crops have measurable biological variation in root and stolon phenotyping and thus may be utilized to identify genomic regions associated with these variations. This is the first comprehensive association mapping study related to potato root and stolon traits. A diverse panel of 192 tetraploid potato (Solanum tuberosum L.) genotypes were grown in aeroponics to reveal a biologically significant variation and detection of genomic regions associated with the root and stolon traits. Phenotyping of root traits was performed by image analysis software “WinRHIZO” (a root scanning method), and stolon traits was measured manually, while SolCAP 25K potato array was used for genotyping. Significant variation was observed between the potato genotypes for root and stolon traits along with high heritabilities (0.80 in TNS to 0.95 in SL). For marker-trait associations, Q + K linear mixed model was implemented and 50 novel genomic regions were detected. Significantly associated SNPs with stolon traits were located on chr 4, chr 6, chr 7, chr 9, chr 11 and chr 12, while those linked to root traits on chr 1, chr 2, chr 3, chr 9, chr 11, and chr 12. Structure and PCA analysis grouped genotypes into four sub-populations disclosing population genetic diversity. LD decay was observed at 2.316 Mbps (r2 = 0.29) in the population. The identified SNPs were associated with genes performing vital functions such as root signaling and signal transduction in stress environments (GT-2 factors, protein kinases SAPK2-like and protein phosphatases “StPP1”), transcriptional and post-transcriptional gene regulation (RNA-binding proteins), sucrose synthesis and transporter families (UGPase, Sus3, SuSy, and StSUT1) and PVY resistance (Ry sto). The findings of our study can be employed in future breeding programs for improvement in potato production.
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Acknowledgements
The Scientific and Technological Research Council of Turkey (TUBITAK) was financially supported this study through Project # 115O949. The current paper belongs to the Ph.D. thesis of the corresponding author. I would also like to thank Eric Kuopuobe Naawe for helping in the maintenance of the aeroponic system during research work. Furthermore, an appreciation was acknowledged by all my colleagues during the phenotyping.
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MEÇ: supervised and designed the study, MEÇ and UD: funding acquisition, MFY: set an experiment and phenotypic data acquisition, MFY and MN: phenotyping and genotyping data curation and analysis, UD: formal computational analysis, MFY: drafted the manuscript, MEÇ, UD, MFY and MN review, editing and approved the manuscript.
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Yousaf, M.F., Demirel, U., Naeem, M. et al. Association mapping reveals novel genomic regions controlling some root and stolon traits in tetraploid potato (Solanum tuberosum L.). 3 Biotech 11, 174 (2021). https://doi.org/10.1007/s13205-021-02727-6
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DOI: https://doi.org/10.1007/s13205-021-02727-6