Abstract
Bacillus velezensis LPL061, which shows strong exopolysaccharide (EPS) producing capacity, was isolated from carnations in Beijing, China. The complete genome of LPL061 comprised a single circular chromosome (3,907,268 bp; G+C content of 46.7%) with 3,737 coding DNA sequences, 26 rRNA, and 89 tRNA. According to genome analysis, 12 protein-coding genes which related to polysaccharide biosynthesis in LPL061 were identified. Comparative genome analysis revealed that the EPS biosynthetic gene cluster was relatively conserved among Bacillus species. EPS showed approximately 60% inhibitory activity on the α-glucosidase at 100 μg/mL. The results of quantitative reverse transcription PCR further demonstrated that compared to insulin-resistant model with insulin (500 μg/mL) (without EPS treatment), the insulin-resistant HepG2 cells treated with EPS decreased the expression of phosphoenolpyruvate carboxykinase (PEPCK) from 4.425 to 0.1587, glucose-6-phosphatase (G6Pase) decreased from 4.272 to 0.1929, and glycogen synthase kinase3β (GSK(3)β) decreased from 2.451 to 0.993, respectively. Meanwhile, EPS treatment increased GS expression and resulted in intracellular glycogen concentration increased from 28.30% to 86.48%, which further supported that EPS form LPL061 could reduce the concentration of blood glucose effectively. These results could be beneficial for better understanding of the hypoglycemic mechanism of B. velezensis LPL061 EPS and developing an EPS-based anti-diabetic agent in the future.
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Data availability
The complete genome sequence of B. velezensis LPL061 was deposited at GenBank under the accession number of CP042271. This strain has been deposited in China Center of Industrial Culture Collection under the accession number of CICC NO. 24192. The community metadata standards the “Minimal Information about any (X) Sequence” (MixS) was shown in Table S1.
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Funding
This project was funded by the National Natural Science Foundation of China (Nos. 31671831 and 31271827), Beijing Innovation Team Project of Sturgeon and Trout (BAIC08-2020).
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RYW and PLL designed the experiments. RYW performed the experiments. RYW, YXQ and QS analyzed the results and wrote the manuscript. All authors read and approved the final manuscript.
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Wu, R., Qin, Y., Shen, Q. et al. The complete genome sequence of Bacillus velezensis LPL061, an exopolysaccharide-producing bacterium. 3 Biotech 10, 243 (2020). https://doi.org/10.1007/s13205-020-02228-y
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DOI: https://doi.org/10.1007/s13205-020-02228-y