Abstract
A novel gene (crc1) from Candida boidinii was cloned and then overexpressed in a recombinant strain BL21(DE3)/pET30a-crc1 of Escherichia coli. The resulting carbonyl reductase was prepared through fermentations using the recombinant strain. The purified enzyme showed an NADPH-dependent activity and specific activity was 4.65 U/mg using t-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate (ATS-6) as substrate. The enzyme was optimally active at 35 °C and pH 7, respectively. The apparent Km and Vmax of the enzyme for ATS-6 are 1.5 mM and 21.1 μmol/min mg, respectively, indicating excellent anti-Prelog stereospecificity. Under the optimum condition, t-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate (ATS-7) was prepared with the enzyme with high d.e. value (99.9%) and good conversion (94%) in 4 h, indicating high stereoselectivity and conversion efficiency in biotransformation of ATS-6 to ATS-7.
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Acknowledgements
This work was financially supported by National Natural Science Foundation of China (nos. 81502421 and 21376217), Huimin Science and Technology Program of Ningbo, China (no. 2015C50042), Natural Science Foundation of Ningbo, China (nos. 2014A610209 and 2014A610214), and Industrial Science and Technology Major Project of Ningbo, China (no. 2017C110017).
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Supplementary Fig. 1—Amino acid sequence alignment of Crc1 from Candida boidinii. Supplementary Fig. 2—Gene cloning, heterologous expression and enzyme purification of the carbonyl reductase. Supplementary Fig. 3—Optimization of Crc1 expression in Escherichia coli BL21(DE3)/pET30a-crc1. Supplementary Fig. 4—Biocatalysis of ATS-6 to ATS-7 by Crc1 (DOC 6464 kb)
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Jin, Q., Wu, Z., Dou, Y. et al. A novel carbonyl reductase with anti-Prelog stereospecificity for the production of t-butyl 6-cyano-(3R, 5R)-dihydroxyhexanoate. 3 Biotech 9, 194 (2019). https://doi.org/10.1007/s13205-019-1722-8
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DOI: https://doi.org/10.1007/s13205-019-1722-8