Abstract
In this study, we evaluated the potential of yeasts isolated from Amazon to produce second-generation ethanol from sugarcane bagasse delignified with alkaline hydrogen peroxide and hydrolysed with commercial enzyme preparation. The best efficiency savings in glucose and release of xylose were determined by considering the solids and enzyme loads. Furthermore, we selected Spathaspora passalidarum UFMG-CM-Y473 strain with the best fermentative parameters. Fermentations used bagasse hydrolysate without any nutritional supplementation, a significant difference from previous studies, which is closer to industrial conditions. Ethanol yield of 0.32 g/g and ethanol productivity of 0.34 g/L h were achieved after the consumption of 78% of the sugar. This hydrolysis/fermentation technology package could represent the input of an additional 3180 L of ethanol per hectare in areas of average sugarcane productivity such as 60 ton/ha. Thus, we concluded that Sp. passalidarum UFMG-CM-Y473 has a clear potential for the production of second-generation ethanol from delignified and enzyme-hydrolysed bagasse.
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Acknowledgements
This work was sponsored by the Bioethanol Research Network of the State of Pernambuco (CNPq-FACEPE/PRONEM APQ-1452-2.01/10 awarded to MAMJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (National Council for Scientific and TechnologicaL Development) (CNPq 552877/2007-7 awarded to BUS) and Fundação do Amparo a Pesquisa do Estado de Minas Gerais (Research Foundation for the State of Minas Gerais) (FAPEMIG—APQ-01347-12 awarded to CAR). The authors would also like to express their thanks to FACEPE and CNPq agencies for their financial support and the Dr. Everardo Sampaio, from the Universidade Federal de Pernambuco, for the suggestions and revision of the manuscript.
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Figure S1. FTIR spectra of sugarcane bagasse before and after pre-treatment with alkaline hydrogen peroxide. (TIF 24 KB)
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de Fátima Rodrigues de Souza, R., Dutra, E.D., Leite, F.C.B. et al. Production of ethanol fuel from enzyme-treated sugarcane bagasse hydrolysate using d-xylose-fermenting wild yeast isolated from Brazilian biomes. 3 Biotech 8, 312 (2018). https://doi.org/10.1007/s13205-018-1340-x
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DOI: https://doi.org/10.1007/s13205-018-1340-x