Skip to main content
SpringerLink
Log in

Spathaspora brasiliensis sp. nov., Spathaspora suhii sp. nov., Spathaspora roraimanensis sp. nov. and Spathaspora xylofermentans sp. nov., four novel d-xylose-fermenting yeast species from Brazilian Amazonian forest

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Four new d-xylose fermenting yeast species of the clade Spathaspora were recovered from rotting-wood samples in a region of Amazonian forest, Northern Brazil. Three species produced unconjugated asci with a single elongated ascospore with curved ends. These species are described as Spathaspora brasiliensis, Spathaspora suhii and Spathaspora roraimanensis. Two isolates of an asexually reproducing species belonging to the Spathaspora clade were also obtained and they are described as Spathaspora xylofermentans. All these species are able to ferment d-xylose during aerobic batch growth in rich YP (1 % yeast extract, 2 % peptone and 2 % D-xylose) medium, albeit with differing efficiencies. The type strains are Spathaspora brasiliensis sp. nov UFMG-HMD19.3 (=CBMAI 1425=CBS 12679), Spathaspora suhii sp. nov. UFMG-XMD16.2 (=CBMAI 1426=CBS 12680), Spathaspora roraimanensis sp. nov. UFMG-XMD23.2 (CBMAI 1427=CBS 12681) and Spathaspora xylofermentans sp. nov. UFMG-HMD23.3 (=CBMAI 1428=CBS 12682).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Alves SL Jr, Herberts RA, Hollatz C, Miletti LC, Stambuk BU (2007) Maltose and maltotriose active transport and fermentation by Saccharomyces cerevisiae. J Am Soc Brew Chem 65:99–104

    CAS  Google Scholar 

  • Amorim HV, Lopes ML, de Castro Oliveira JV, Buckeridge MS, Goldman GH (2011) Scientific challenges of bioethanol production in Brazil. Appl Microbiol Biotechnol 91:1267–1275

    Article  PubMed  CAS  Google Scholar 

  • Barbosa AC, Cadete RM, Gomes FCO, Lachance MA, Rosa CA (2009) Candida materiae sp. nov., a yeast species isolated from rotting wood in the Atlantic Rain Forest. Int J Syst Evol Microbiol 59:2104–2106

    Article  PubMed  Google Scholar 

  • Boonmak C, Limtong S, Jindamorakot S, Am-In S, Yongmanitchai W, Suzuki K, Nakase T, Kawasaki H (2011) Candida xylanilytica sp. nov., a xylan-degrading yeast species isolated from Thailand. Int J Syst Evol Microbiol 61:1230–1234

    Article  PubMed  CAS  Google Scholar 

  • Cadete RM, Santos RO, Melo MA, Mouro A, Gonçalves DL, Stambuk BU, Gomes FCO, Lachance MA, Rosa CA (2009) Spathaspora arborariae sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil. FEMS Yeast Res 9:1338–1342

    Article  PubMed  CAS  Google Scholar 

  • Cheng JJ, Timilsina GR (2011) Status and barriers of advanced biofuel technologies: a review. Renew Energ 36:3541–3549

    Article  CAS  Google Scholar 

  • da Cunha-Pereira F, Hickert LR, Sehnem NT, de Souza-Cruz PB, Rosa CA, Ayub MAS (2011) Conversion of sugars present in rice hull hydrolysates into ethanol by Spathaspora arborariae, Saccharomyces cerevisiae, and their co-fermentations. Biores Technol 102:4218–4225

    Article  Google Scholar 

  • Hahn-Hägerdal B, Galbe M, Gorwa-Grauslund MF, Lidén G, Zacchi G (2006) Bio-ethanol—the fuel of tomorrow from the residues of today. Trends Biotechnol 24:549–556

    Article  PubMed  Google Scholar 

  • Hahn-Hägerdal B, Karhumaa K, Fonseca C, Spencer-Martins I, Gorwa-Grauslund MF (2007) Towards industrial pentose-fermenting yeast strains. Appl Microbiol Biotechnol 74:937–953

    Article  PubMed  Google Scholar 

  • Hou X (2012) Anaerobic xylose fermentation by Spathaspora passalidarum. Appl Microbiol Biotechnol 94:205–214

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP, Fell JW, Boekhout T, Robert V (2011a) Methods for isolation phenotypic characterization and maintenance of yeasts. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts: a taxonomic study, 5th edn. Elsevier, New York, pp 87–110

    Chapter  Google Scholar 

  • Kurtzman CP, Fell JW, Boekhout T (2011b) The yeasts: a taxonomic study, 5th edn. Elsevier, Amsterdam

    Google Scholar 

  • Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP (2011) Candida Berkhout, (1923). In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts: a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 987–1278

    Chapter  Google Scholar 

  • Long TM, Su YK, Headman J, Higbee A, Willis LB, Jeffries TW (2012) Cofermentation of glucose, xylose, and cellobiose by the beetle-associated yeast, Spathaspora passalidarum. Appl Environ Microbiol 78:5492–5500

    Article  PubMed  CAS  Google Scholar 

  • Marinoni G, Lachance MA (2004) Speciation in the large-spored Metschnikowia clade and establishment of a new species, Metschnikowia borealis comb. nov. FEMS Yeast Res 4:587–596

    Article  PubMed  CAS  Google Scholar 

  • Merico A, Sulo P, Piskur J, Compagno C (2007) Fermentative lifestyle in yeasts belonging to the Saccharomyces complex. FEBS J 274:976–989

    Article  PubMed  CAS  Google Scholar 

  • Miller GL (1959) Use of dinitrosalycilic acid reagent for determination of reducing sugar. Anal Chem 31:426–429

    Article  CAS  Google Scholar 

  • Nguyen NH, Suh SO, Marshall CJ, Blackwell M (2006) Morphological and ecological similarities: wood-boring beetles associated with novel xylose-fermenting yeasts, Spathaspora passalidarum gen. sp. nov. and Candida jeffriesii sp. nov. Mycol Res 110:1232–1241

    Article  PubMed  Google Scholar 

  • Nguyen NH, Suh S-O, Blackwell M (2011) Spathaspora Nguyen, Suh and Blackwell. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts: a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 795–797

    Chapter  Google Scholar 

  • Norvell LL (2011) Fungal nomenclature. 1.Melbourne approves a new code. Mycotaxon 116:481–490

    Article  Google Scholar 

  • Rozpędowska E, Galafassi S, Johansson L, Hagman A, Piškur J, Compagno C (2011) Candida albicans—a pre-whole genome duplication yeast—is predominantly aerobic and a poor ethanol producer. FEMS Yeast Res 11:285–291

    Article  PubMed  Google Scholar 

  • Solomon BD (2010) Biofuels and sustainability. Ann NY Acad Sci 1185:119–134

    Article  PubMed  Google Scholar 

  • Stambuk BU, Franden MA, Singh A, Zhang M (2003) d-Xylose transport by Candida succiphila and Kluyveromyces marxianus. Appl Biochem Biotechnol 105:255–263

    Article  PubMed  Google Scholar 

  • Stambuk BU, Eleutherio ECA, Florez-Pardo LM, Souto-Maior AM, Bon EPS (2008) Brazilian potential for biomass ethanol: challenge of using hexose and pentose co-fermenting yeast strains. J Sci Ind Res 67:918–926

    CAS  Google Scholar 

  • Suh S-O, Marshall CJ, McHugh JV, Blackwell M (2003) Wood ingestion by passalid beetles in the presence of xylose-fermenting gut yeasts. Mol Ecol 12:3137–3145

    Article  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  • Wohlbach DJ, Kuo A, Sato TK, Potts KM, Salamov AA, Labutti KM, Sun H, Clum A, Pangilinan JL, Lindquist EA, Lucas S, Lapidus A, Jin M, Gunawan C, Balan V, Dale BE, Jeffries TW, Zinkel R, Barry KW, Grigoriev IV, Gasch AP (2011) Comparative genomics of xylose-fermenting fungi for enhanced biofuel production. Proc Natl Acad Sci USA 108:13212–13217

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by grants and fellowships from the Brazilian agencies Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, process no. 552877/2007-7, 551392/2010-0, 551245/2010-7, 560715/2010-2, and 490029/2009-4), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Procad-NF, process no. 2280/2008), Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP/BIOEN/FAPEMIG, process no. 2008/57926-4), Fundação de Apoio à Pesquisa Científica e Tecnológica do Estado de Santa Catarina (FAPESC, process no. 17293/2009-6), and the Natural Science and Engineering Research Council of Canada (M.-A. L.).

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Departamento de Microbiologia, Instituto de Ciências Biológicas (ICB), C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

    Raquel M. Cadete, Monaliza A. Melo & Carlos A. Rosa

  2. Embrapa Agrobiologia, Rodovia BR 465, km 7, Seropédica, RJ, 23890-000, Brazil

    Jerri E. Zilli

  3. Departamento de Biologia, Universidade Federal de Roraima - Campus do Paricarana, Boa Vista, RR, 69310-270, Brazil

    Marcos J. S. Vital

  4. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil

    Adriane Mouro, Alice H. Prompt & Boris U. Stambuk

  5. Departamento de Química, Centro Federal de Educação Tecnológica de Minas Gerais, Belo Horizonte, MG, Brazil

    Fátima C. O. Gomes

  6. Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

    Marc-André Lachance

Authors
  1. Raquel M. Cadete
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Monaliza A. Melo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jerri E. Zilli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marcos J. S. Vital
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Adriane Mouro
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Alice H. Prompt
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fátima C. O. Gomes
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Boris U. Stambuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Marc-André Lachance
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Carlos A. Rosa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Carlos A. Rosa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cadete, R.M., Melo, M.A., Zilli, J.E. et al. Spathaspora brasiliensis sp. nov., Spathaspora suhii sp. nov., Spathaspora roraimanensis sp. nov. and Spathaspora xylofermentans sp. nov., four novel d-xylose-fermenting yeast species from Brazilian Amazonian forest. Antonie van Leeuwenhoek 103, 421–431 (2013). https://doi.org/10.1007/s10482-012-9822-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-012-9822-z

Keywords

Search

Navigation