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Molecular characterization of freshwater microalgae and nutritional exploration to enhance their lipid yield

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Abstract

Biomass and lipid yield under nutrient depleted and supplemented conditions of N and Mg in two freshwater isolates, Chlorella sp. NC-MKM and Scenedesmus acutus NC-M2 from Meghalaya were investigated for biodiesel production. Both the strains, Chlorella sp. NC-MKM and Scenedesmus acutus NC-M2 are oleaginous in nature having lipid content of 40.2 ± 1.4 and 37.3 ± 2.6% DCW, respectively. The significant increase (92.8%) in lipid content was found in N-depleted condition while an increase (46.65%) in biomass yield was observed under Mg-supplemented condition in Chlorella sp. NC-MKM. Studying the interactive effects of nutrient depletion and supplementation, combination of N-depleted and Mg-supplemented condition was selected for further investigation to check enhanced lipid yield in Chlorella sp. NC-MKM. The results showed a significant increase in biomass yield, lipid yield and lipid content (30, 66.8, and 28.66%, respectively). Under this condition, accumulation of neutral lipid was also enhanced (47.17% M2 gated cells) compared to control (21.37% M2 gated cells). Further, FAMEs revealed that the relative percentage of saturated and mono-unsaturated fatty acids increased (66.16%) in Chlorella sp. NC-MKM compared to control that improves biodiesel properties.

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References

  • Belotti G, Bravi M, de Caprariis B, de Filippis P, Scarsella M (2013) Effect of nitrogen and phosphorus starvations on Chlorella vulgaris lipids productivity and quality under different trophic regimens for biodiesel production. Am J Plant Sci 4:44–51

    Article  CAS  Google Scholar 

  • Chen W, Zhang C, Song L, Sommerfeld M, Hu Q (2009) A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J Microbiol Methods 77:41–47

    Article  CAS  PubMed  Google Scholar 

  • Darzins AI, Pienkos P, Edye L (2010) Current status and potential for algal biofuels production: a report to IEA Bioenergy Task 39 commercializing liquid biofuels from biomass. International Energy Agency Bioenergy Task 39

  • Deng X, Fei X, Li Y (2011) The effects of nutritional restriction on neutral lipid accumulation in Chlamydomonas and Chlorella. Afr J Microbiol Res 5:260–270

    CAS  Google Scholar 

  • El-Kassas HY (2013) Growth and fatty acid profile of the marine microalga Picochlorum sp. grown under nutrient stress conditions. Egypt J Aquat Res 39:233–239

    Article  Google Scholar 

  • Fan J, Cui Y, Wan M, Wang W, Li Y (2014) Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors. Biotechnol Biofuels 7:17–31

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Finkle BJ, Appleman D (1953) The effect of magnesium concentration on growth of Chlorella. Plant Physiol 28:664–673

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Francisco EC, Neves DB, Jacob-Lopes E, Franco TF (2010) Microalgae as feedstock for biodiesel production: carbon dioxide sequestration, lipid production and biofuel quality. J Chem Technol Biotechnol 85:395–403

    Article  CAS  Google Scholar 

  • Gopinath A, Puhan S, Nagarajan G (2009) Relating the cetane number of biodiesel fuels to their fatty acid composition: a critical study. Proc Inst Mech Eng Part D: J Automob Eng 223:565–583

    Article  Google Scholar 

  • Gorain PC, Bagchi SK, Mallick N (2013) Effects of calcium, magnesium and sodium chloride in enhancing lipid accumulation in two green microalgae. Environ Technol 34:1887–1894

    Article  CAS  PubMed  Google Scholar 

  • Gwak Y, Hwang Y, Wang B, Kim M, Jeong J, Lee CG, Hu Q, Han D, Jin E (2014) Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis. J Exp Bot 65:4317–4334

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hepbasli A (2008) A key review on energetic analysis and assessment of renewable energy resources for a sustainable future. Renew Sustain Energy Rev 12:593–661

    Article  Google Scholar 

  • Hsieh CH, Wu WT (2009) Cultivation of microalgae for oil production with a cultivation strategy of urea limitation. Bioresour Technol 100:3921–3926

    Article  CAS  PubMed  Google Scholar 

  • Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639

    Article  CAS  PubMed  Google Scholar 

  • Huang GH, Chen G, Chen F (2009) Rapid screening method for lipid production in alga based on Nile red fluorescence. Biomass Bioenerg 33:1386–1392

    Article  CAS  Google Scholar 

  • Islam MA, Magnusson M, Brown RJ, Ayoko GA, Nabi MN, Heimann K (2013) Microalgal species selection for biodiesel production based on fuel properties derived from fatty acid profiles. Energies 6:5676–5702

    Article  CAS  Google Scholar 

  • Knothe G (2005) Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters. Fuel Process Technol 86:1059–1070

    Article  CAS  Google Scholar 

  • Knothe G (2006) Analyzing biodiesel standards and other methods. J Am Oil Chem Soc 83:823–833

    Article  CAS  Google Scholar 

  • Krisnangkura K (1986) A simple method for estimation of cetane index of vegetable oil methyl esters. J Am Oil Chem Soc 63:552–553

    Article  CAS  Google Scholar 

  • Ladommatos N, Parsi M, Knowles A (1996) The effect of fuel cetane improver on diesel pollutant emissions. Fuel 75:8–14

    Article  CAS  Google Scholar 

  • Li Y, Mu J, Chen D, Han F, Xu H, Kong F, Xie F, Feng B (2013) Production of biomass and lipid by the microalgae Chlorella protothecoides with heterotrophic Cu(II) stressed (HCuS) coupling cultivation. Bioresour Technol 148:283–292

    Article  CAS  PubMed  Google Scholar 

  • Li YX, Zhao FJ, Yu DD (2015) Effect of nitrogen limitation on cell growth, lipid accumulation and gene expression in Chlorella sorokiniana. Braz Arch Biol Technol 58:462–467

    Article  CAS  Google Scholar 

  • Li Huang J, Xu T, Li L, Wang T, Deng XY (2014) Effects of additional Mg2+ on the growth, lipid production, and fatty acid composition of Monoraphidium sp. FXY-10 under different culture conditions. Ann Microbiol 64:1247–1256

    Article  CAS  Google Scholar 

  • Malcata FX (2011) Microalgae and biofuels: a promising partnership? Trends Biotechnol 29:542–549

    Article  CAS  PubMed  Google Scholar 

  • Mandal S, Mallik N (2009) Microalga Scenedesmus obliquus as a potential source for biodiesel production. Appl Microbiol Biotechnol 84:281–291

    Article  CAS  PubMed  Google Scholar 

  • Mata TM, Almeida R, Caetano NS (2013) Effect of the culture nutrients on the biomass and lipid productivities of microalgae Dunaliella tertiolecta. Chem Eng Trans 32:973–978

    Google Scholar 

  • Metzger P, Largeau C (2005) Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Appl Microbiol Biotechnol 66:486–496

    Article  CAS  PubMed  Google Scholar 

  • Moon-van der Staay SY, Gerog WM, Guillou L, Vaulot D (2000) Abundance and diversity of prymnesiophytes in the picoplankton community from the equatorial Pacific Ocean inferred from 18S rDNA sequences. Limnol oceanogr 45:98–109

    Article  CAS  Google Scholar 

  • Parmar A, Singh NK, Pandey A, Gnansounou E, Madamwar D (2011) Cyanobacteria and microalgae: a positive prospect for biofuels. Bioresour Technol 102:10163–10172

    Article  CAS  PubMed  Google Scholar 

  • Praveenkumar R, Shameera K, Mahalakshmi G, Akbarsha MA, Thajuddin N (2012) Influence of nutrient deprivations on lipid accumulation in a dominant indigenous microalga Chlorella sp., bum 110088: evaluation for biodiesel production. Biomass Bioenerg 37:60–66

    Article  CAS  Google Scholar 

  • Ramírez-Verduzco LF, Rodríguez-Rodríguez JE, Jaramillo-Jacob AR (2012) Predicting cetane number, kinematic viscosity, density and higher heating value of biodiesel from its fatty acid methyl ester composition. Fuel 91:102–111

    Article  CAS  Google Scholar 

  • Ramos MJ, Fernandez CM, Casas A, Rodriguez L, Perez A (2009) Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour Technol 100:261–268

    Article  CAS  PubMed  Google Scholar 

  • Rippka R, Herdman H (1992) Pasteur culture collection of cyanobacteria catalogue & taxonomic handbook. 1. Catalogue of strains. Institut Pasteur, Paris

    Google Scholar 

  • Rismani-Yazdi H, Haznedaroglu BZ, Hsin C, Peccia J (2012) Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation. Biotechnol Biofuels 5:74

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Satpati GG, Pal R (2015) Rapid detection of neutral lipid in green microalgae by flow cytometry in combination with Nile red staining—an improved technique. Ann Microbiol 65:937–949

    Article  CAS  Google Scholar 

  • Sharma AK, Sahoo PK, Singhal S, Patel A (2016) Impact of various media and organic carbon sources on biofuel production potential from Chlorella spp. 3 Biotech 6:116

    Article  PubMed  PubMed Central  Google Scholar 

  • Sheehan J, Dunahay T, Benemann J, Roessler P (2011) A look back at the U.S. Department of Energy’s aquatic species program: biodiesel from algae. NREL/TP-580-24190. U.S. Department of Energy’s Office of Fuels Development, Golden

    Google Scholar 

  • Soylu EN, Gonulol A (2012) Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kizilirmak Delta. Turk J Biol 36:247–254

    Google Scholar 

  • Sun X, Cao Y, Xu H, Liu Y, Sun J, Qiao D, Cao Y (2014) Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process. Bioresour Technol 155:204–212

    Article  CAS  PubMed  Google Scholar 

  • Sydney EB, Sturm W, de Carvalho JC, Thomaz-Soccol V, Larroche C, Pandey A, Soccol CR (2010) Potential carbon dioxide fixation by industrially important microalgae. Bioresour Technol 101:5892–5896

    Article  CAS  PubMed  Google Scholar 

  • Tale M, Ghosh S, Kapandnis B, Kale S (2014) Isolation and characterization of microalgae for biodiesel production from Nisargruna biogas plant effluent. Bioresour Technol 169:328–335

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6 (2013) Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Turpin DH (1991) Effects of inorganic N availability on algal photosynthesis and carbon metabolism. J Phycol 27:14–20

    Article  CAS  Google Scholar 

  • Ulloa G, Otero A, Sánchez M, Sineiro J, Núñez MJ, Fábregas J (2012) Effect of Mg, Si, and Sr on growth and antioxidant activity of the marine microalga Tetraselmis suecica. J Appl Phycol 24:1229–1236

    Article  CAS  Google Scholar 

  • Wagenen JV, Miller TW, Hobbs S, Hook P, Crowe B, Huesmann M (2012) Effect of temperature and light on fatty acid production in Nannochloropsis salina. Energies 5:731–740

    Article  CAS  Google Scholar 

  • Xin L, Hong-ying H, Ke G, Ying-xue S (2010) Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Bioresour Technol 101:5494–5500

    Article  CAS  PubMed  Google Scholar 

  • Yeh KL, Chang JS (2011) Nitrogen starvation strategies and photobioreactor design for enhancing lipid production of a newly isolated microalga Chlorella vulgaris ESP-31: implications for biofuels. Biotechnol J 6:1358–1366

    Article  CAS  PubMed  Google Scholar 

  • Zhang L, Pei H, Chen S, Jiang L, Hou Q, Yang Z, Yu Z (2017) Salinity-induced cellular cross-talk in carbon partitioning reveals starch-to-lipid biosynthesis switching in low-starch freshwater algae. Bioresour Technol 250:449–456

    Article  CAS  PubMed  Google Scholar 

  • Zhu F, Massana R, Not F, Marie D, Vaulot D (2005) Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene. FEMS Microbiol Ecol 52:79–92

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

Financial support from DBT and CSIR, Government of India has been thankfully acknowledged. Authors are thankful to the Head of department, Biotechnology and Bioinformatics, North-Eastern Hill University for providing the necessary facilities. Also, authors thank to Guwahati Biotech Park, Assam, India for support in GC-MS analysis.

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  1. Environmental Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, 793022, India

    Madan Kumar Mandal & Neha Chaurasia

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  1. Madan Kumar Mandal
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Correspondence to Neha Chaurasia.

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Mandal, M.K., Chaurasia, N. Molecular characterization of freshwater microalgae and nutritional exploration to enhance their lipid yield. 3 Biotech 8, 238 (2018). https://doi.org/10.1007/s13205-018-1248-5

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