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Regulatory genes and their roles for improvement of antibiotic biosynthesis in Streptomyces

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Abstract

The numerous secondary metabolites in Streptomyces spp. are crucial for various applications. For example, cephamycin C is used as an antibiotic, and avermectin is used as an insecticide. Specifically, antibiotic yield is closely related to many factors, such as the external environment, nutrition (including nitrogen and carbon sources), biosynthetic efficiency and the regulatory mechanisms in producing strains. There are various types of regulatory genes that work in different ways, such as pleiotropic (or global) regulatory genes, cluster-situated regulators, which are also called pathway-specific regulatory genes, and many other regulators. The study of regulatory genes that influence antibiotic biosynthesis in Streptomyces spp. not only provides a theoretical basis for antibiotic biosynthesis in Streptomyces but also helps to increase the yield of antibiotics via molecular manipulation of these regulatory genes. Currently, more and more emphasis is being placed on the regulatory genes of antibiotic biosynthetic gene clusters in Streptomyces spp., and many studies on these genes have been performed to improve the yield of antibiotics in Streptomyces. This paper lists many antibiotic biosynthesis regulatory genes in Streptomyces spp. and focuses on frequently investigated regulatory genes that are involved in pathway-specific regulation and pleiotropic regulation and their applications in genetic engineering.

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Acknowledgements

This work is supported by the Open Research Program of Key Laboratory of Synthetic Biology, the Chinese Academy of Sciences (SYN201612) and the Key Program of Sichuan Science and Technology Project (2017GZ0430). The authors thank all the supporting institutions.

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Authors and Affiliations

  1. Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education and College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, Sichuan, China

    Fengjuan Lu, Yanyan Hou, Heming Zhang & Yongqiang Tian

  2. Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan, China

    Yiwen Chu

  3. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

    Haiyang Xia

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  1. Fengjuan Lu
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  2. Yanyan Hou
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  3. Heming Zhang
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  4. Yiwen Chu
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  5. Haiyang Xia
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Correspondence to Yongqiang Tian.

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This study was funded by the Open Research Program of Key Laboratory of Synthetic Biology, the Chinese Academy of Sciences (SYN201612) and the Key Program of Sichuan Science and Technology Project (2017GZ0430).

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Lu, F., Hou, Y., Zhang, H. et al. Regulatory genes and their roles for improvement of antibiotic biosynthesis in Streptomyces . 3 Biotech 7, 250 (2017). https://doi.org/10.1007/s13205-017-0875-6

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