Abstract
In this study, a novel nano-biosensor for uric acid detection has been designed and fabricated by embedding uricase into a copper nanozyme, which possessed outstanding peroxidase-like activity and could efficiently mimic horseradish peroxidase (HRP). After optimizing the preparation conditions and characterization, we successfully applied the nano-biosensor to detect uric acid levels. The equation of linear regression was prescribed as y = 0.0064x + 0.3299, while its correlation coefficient is 0.9886. The colorimetric sensor exhibited a linear response range over 1–50 μM and a relatively low limit of detection (LOD) of 0.6 µM. The nano-biosensor has the advantages of easy preparation, good reusability and excellent storage stability, while this one-step colorimetric method is simple to be operated and has high selectivity for detection of the uric acid levels in actual human samples.
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Acknowledgements
The authors are grateful for the financial assistance provided by Science and technology development program of Jilin Province (No. 20200301029RQ), Jilin COFCO Biochemical Co., Ltd. (2018220002000466), and the Fund of Scientific Research from the Education Department of Jilin Province (JJKH20210170KJ).
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Chi, X., Cheng, Q., Yang, K. et al. Fabrication of a novel nano-biosensor for efficient colorimetric determination of uric acid. Appl Nanosci 12, 2255–2264 (2022). https://doi.org/10.1007/s13204-022-02498-3
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DOI: https://doi.org/10.1007/s13204-022-02498-3