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Deep eutectic solvent-assisted facile synthesis of copper hydroxide nitrate nanosheets as recyclable enzyme-mimicking colorimetric sensor of biothiols

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Abstract

In the quest for alternative products that would conquer natural enzyme drawbacks, enzyme-like nanomaterials with controllable morphology, high catalytic activity, excellent stability, and reusability have gained extensive attention in recent years. Herein, a simple and versatile strategy based on basic deep eutectic solvents was used to create layered copper hydroxide nitrate (Cu2(OH)3NO3) with a well-structured nanosheet-like morphology. The present nanosheets exhibited extraordinary oxidase and peroxidase-like activity. More importantly, these nanosheets have shown the ability to operate at low and high temperatures with appreciable stability and multiple reusabilities. Based on inhibiting the oxidase-like activity of the prepared Cu2(OH)3NO3, we designed a colorimetric sensing technique with a high-efficiency detection of biothiols in serum samples. Because of the simplicity and low-cost fabrication approach, our findings would be beneficial to the artificial enzyme research community as another facile and green tactics to fabricate heterogeneous artificial enzymes.

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Acknowledgments

The authors gratefully acknowledge the National Natural Science Foundation of China (No. 21822407), the National Key Research and Development Program of China (2019YFC1905501, 2019YFD1002403), and the Chinese Academy of Sciences-the World Academy of Sciences (CAS-TWAS) President’s Fellowship Programme.

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Authors and Affiliations

  1. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, Gansu, China

    Jean Claude Munyemana, Jia Chen, Xin Wei, Mohammad Chand Ali, Yangxia Han & Hongdeng Qiu

  2. University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, 100039, China

    Jean Claude Munyemana & Hongdeng Qiu

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  1. Jean Claude Munyemana
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  2. Jia Chen
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  6. Hongdeng Qiu
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Correspondence to Jia Chen or Hongdeng Qiu.

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The study using biological samples has been approved by the Ethics Committees of Gansu Provincial Hospital and Lanzhou Institute of Chemical Physics and has been performed in accordance with the ethical standards.

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Munyemana, J.C., Chen, J., Wei, X. et al. Deep eutectic solvent-assisted facile synthesis of copper hydroxide nitrate nanosheets as recyclable enzyme-mimicking colorimetric sensor of biothiols. Anal Bioanal Chem 412, 4629–4638 (2020). https://doi.org/10.1007/s00216-020-02712-7

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