Abstract
Prevention of infection at the surgical area and outbreak of wounds are the most important and at the same time challenging tasks in clinical care. This research investigates the healing effect of laparotomy wounds after the use of bio-fabricated gold nanoparticles (Au NPs) prepared utilizing Thuja occidentalis leaf extract. Ultraviolet–visible (UV–Vis) spectroscopy and X-ray diffraction (X-RD) results confirmed the successful formation of Au NPs. High-resolution Transmission Electron Microscopy (HR-TEM) images confirmed the formation of spherical shaped Au NPs comprising of 30–50 nm particle size. Dermal fibroblasts were subjected to increased concentrations of Au NPs and their effect of cytotoxicity and synthesis of collagen was evaluated. The procedure of celiotomy was conducted on rabbits where the surgical area was exposed topically with either vehicle or once/everyday with Au NPs for about 14 days. Au NPs raised the collagen production from dermal fibroblasts and improved the expression of caspase 3 by exposure to a long period. AuNPs also showed cytotoxic impact with half-maximal inhibitory concentration (IC50) of 0.16 mg/mL. Regular treatment of surgical area with AuNPs led to higher collagen deposition with reduced microbial load and enhanced healing of wounds.
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Dong, Y., Hu, XM., Cao, YF. et al. Thuja occidentalis mediated AuNPs as wound dressing agents for abdominal wound healing in nursing care after surgery. Appl Nanosci 10, 3577–3584 (2020). https://doi.org/10.1007/s13204-020-01459-y
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DOI: https://doi.org/10.1007/s13204-020-01459-y