Abstract
Egyptian Conocarpus erectus L., or the buttonwood, was studied in different terms. This study used light and scanning electron microscopy, HPLC, and GC–MS analyses. Foliar morphology, anatomy of stems and leaves, pollen morphology, and phytochemical and endofungal attributes were investigated. It was an evergreen shrub with alternate leaves, a pair of nectary glands on the leaf base, and a few indumentums. Trichomes of both non- and glandular hairs were found. Stomata were sized, reaching 37.5 × 25.0 µm. They appeared on both abaxial and adaxial surfaces. The stem and leaf interior structure was typically like most dicots. Their internal structure revealed the mycelium of Aspergillus flavus throughout. Similarly, the accumulation of phenolic compounds as colored particles and integrated phytochemicals in crystalloid druses are observed internally. The endophytic fungus A. flavus, isolated from the leaves of C. erectus, contained the highest concentration of kojic acid. As a result, we could consider this A. flavus strain as a source of kojic acid for application in industry. Fungal and C. erectus extracts showed broad-range antifungal activity against three opportunistic human pathogenic fungi (Geotrichum candidum OL960606, Neoscytalidium dimidiatum OL960610, and Scopulariopsis coprophila OL960621). The major bioactive compounds of C. erectus leaf extracts were gallic acid, rutin, and rosmarinic acid. In conclusion, the endophytic A. flavus isolated from C. erectus has a variety of biological and medicinal uses that make it a potential commercial resource.
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1 Introduction
Conocarpus erectus L. belongs to the family Combretaceae R.Br. (Bashir et al. 2015). It is an evergreen shrub known as buttonwood or button mangrove because its fruits resemble buttons. It frequently develops and spreads quickly in environments with poor soil fertility, elevated temperatures, poor drainage, and high saline levels (Gilman and Watson 1993; Nelson 1996; El-Mahrouk et al. 2010).
According to Stace (2007), Conocarpus is a genus that can be found in West Africa and both the East and West coasts of America, in addition to the non-mangrove member of the genus (C. lancifolius) that is found in northeast Africa and Arabia. Its hardwood is used in manufacturing furniture, fire, and charcoal (Al-Wabel et al. 2015). In the West Indies, Conocarpus is highly valuable for fuel (Stace 2007). The species erectus lives on Earth in tropical and subtropical locations (Schoener 1988; Rosa Galdino Bandeira 2003; Abdel-Hameed et al. 2012). However, C. erectus does start to have some ecological and environmental relevance. It might rebuild dunes, provide food and refuge for various species, protect the soil from storm damage, and restore dunes (Popp et al. 1989).
Conocarpus erectus's general vegetative features and internal structure are rarely discussed in the literature. Stace (2007) presented a proper identification based on its habits, distribution, morphology, palynology, and other features. Recently, the structure of pollen grains in C. erectus has been studied by Namjoyan et al. (2020). Earlier, Bailey (1976) described C. erectus as an evergreen tree up to 6 m high with a spreading crown, grey or brown bark, medium-green leaves, and greenish flowers in dense cone-like heads in terminal panicles. El-Mahrouk et al. (2010) studied the standard internal structure of the leaves and stem of C. erectus in comparison with irrigation with diluted seawater. However, crucial taxonomic and characterization data can be obtained from the macro- and micro-morphological features of the plant's vegetative or reproductive parts. According to Afifi et al. (2021), Conocarpus is a genus of evergreen trees endemic to several habitats and tolerant of salt, heat, and drought. The results of that study demonstrated the production of polyphenol compounds by various plant parts of C. erectus and C. lancifolius. They also highlighted the unusual source of Conocarpus species for bioactive substances that can be utilized for pharmacological indications and the production of functional food. Conocarpus spp. found to include vanillic acid, p-coumaric acid, quercetin, rutin hydrate, flavone, t-ferulic acid, sinapic acid, and protocatechuic acid.
Aziz et al. (2018) showed that medicinal plants are functional food and medicine sources, especially in underdeveloped areas. Ali (2018) noted the presence of many natural plant compounds in C. erectus L. Polyphenols, including flavonoids and tannins, can be found. According to Koche et al. (2016), phenolics are a class of natural compounds with significant implications for medical practice. The antioxidant, anticancer, cytotoxic, antibacterial, and vasodilatory properties of phenolics are well documented. High hepatoprotective, antioxidant, and anticancer activity were observed in C. erectus (Shohayeb et al. 2013). This plant has also been found to have variable pharmacologically active phytochemicals, e.g., conocarpan, conocarpol, gallic acid, ellagic acid, ellagitannin, castalagin, quercetin, myricetin, syringetin, etc. (Sabi-ur-Rehman et al. 2019). This plant may have a significant role in preventing and curating diseases for which oxidants or free radicals are responsible (Abdel-Hameed et al. 2013).
Traditionally, people can use it for treating some disorders such as orchitis, prickly heat, headache, anemia, bleeding, catarrh, diabetes, diarrhea, conjunctivitis, tumors, gonorrhea, syphilis, and as an antipyretic and anti-inflammatory in the treatment of fever and swellings (Nascimento et al. 2016; Raza et al. 2016, 2018). Its bark and fruits manage diabetes, hemorrhoids, and wounds (Santos et al. 2018). The WHO revealed that various drugs are best obtained from medicinal plants.
Endophytic fungi, often known as plant-associated fungi, are a diverse group of microorganisms typically found asymptomatically in plant tissues or intercellular gaps. Some authors used different microscopy techniques to illustrate the occurrence of endofungi within the histological or anatomical structure of host plants (Salgado et al. 2007; Bernardi-Wenzel et al. 2010). Endophytes assist plants with several physiological processes, such as growth, reproduction, senescence, and others (Wilson 1995). Since endophytes live within the host plant, it is believed that they help the host plant respond to biotic and abiotic stress by increasing the host plant's tolerance to things like drought, salt, low temperatures, etc. (Arachevaleta et al. 1989; Wen et al. 2022). As a result of the mutualistic co-evolution of plants and their endophytes, several complex biological substances known as secondary metabolites have been produced. Endophytes may be used as alternative sources for these metabolites because several of them have been found to produce chemicals with antimicrobial properties (Tan and Zou 2001).
The ability of kojic acid to suppress tyrosinase, the enzyme responsible for hyperpigmentation, has led to its inclusion in various cosmetic formulations, such as creams, lotions, and soaps, to mitigate the wrinkle effects of sun exposure. Saeedi et al. (2019) noted that it is also used to avoid the browning of food. This metabolite and its derivatives are in high demand due to their potential utility and widespread use in the cosmetics, personal care, food, agricultural, pharmaceutical, medical, and chemical industries. Aspergillus oryzae and Aspergillus flavus frequently produce kojic acid (Ola et al. 2019).
The authors of this study intended to evaluate and introduce some beneficial morphological and palynological characteristics of C. erectus in Egypt. To demonstrate the histological presence of this species' endophytes, which may produce active chemical compounds with potential therapeutic applications. Also, to illustrate the anatomical structure of the stems and leaves of C. erectus.
2 Material and methods
2.1 Plant material
Data for this study of the Egyptian species C. erectus L. were based on collected specimens from the campus of South Valley University (SVU), Qena Governorate, Egypt. Collections were gathered from two sites with the coordinates [Latitude 26.19208o and Longitude 32.74527o]. Voucher specimens and permanent slides were deposited in the South Valley University-Qena Herbarium (QNA, a proposed acronym). Voucher specimens and slides were investigated and photographed by the author. According to Stace (2007) and the website https://wfoplantlist.org, nomenclature and synonyms were reviewed for taxonomic attributes.
2.2 Methods
Plant parts were evaluated by neck eyes. Measurements were taken with a ruler directly or, for finer details, by an ocular piece under a dissecting microscope.
2.3 Light microscopy investigations (LM)
An Olympus-light dissecting microscope was used to study vegetative leaves and the stem. This microscope has an ocular-equipped × 20 eyepiece. The ocular piece was calibrated by a standard graduated slide [graduation equals 1 mm (about 0.04 in)]. Digital photographs were captured using the Samsung ST72 HD digital camera.
Good leaf portions were inserted in test tubes and treated with lactic acid 88% in a water bath at 100 °C to remove the cuticle and soften the leaf tissue to investigate foliar micromorphology (Clarke 1960). This made it simpler to prepare slides with Canada balsam-mounted epidermal layers from the leaf's abaxial and adaxial sides. A study was done on stomata features and trichome types (Table 1).
Only young, robust leaves and stems were chosen for the anatomical studies. Plant parts were collected from the fifth node upwards on the vegetative stems and leaves. They were preserved in 70% alcohol and dehydrated in a series of ethyl alcohols; the fresh specimens were immersed in a formalin-acetic acid alcohol (FAA) solution for at least 48 h (about two days). The sections were cut using a rotary microtome, dyed with safranin fast green, and mounted in Canada balsam (Johansen 1940). A Labomed-Labo America, Inc. LM was used to analyze sections of permanent slides at magnifications of 4, 10, and 40 to provide the best possible clarity. ToupView digital camera attached to Leica DM1000 microscope was used for microphotography. Table 2 lists the values found for the various anatomical characteristics.
Samples from the mature anthers of the flowers were collected for palynology research. The sample was placed on a clean slide, briefly brought to a boil in water, rehydrated in a 10% KOH aqueous solution, mounted in glycerin jelly, and sealed with paraffin wax. Using a light microscope (Labomed-Labo America, Inc.) with a magnification of 100, permanent slides were inspected. Micrographs were taken using a Leica DM1000 microscope with a ToupTek ToupView digital camera. Using ocular tools, pollen grain measurements were based on an average of 15–20 readings for each P- and E-diameter for each sample of 10–15 pollen grains (Table 1).
2.4 Scanning electron microscopy investigations (SEM)
Healthy leaf portions were selected for trichome and stomata examination, mounted onto clean, marked holders using double-sided self-adhesive tape, scanned, and photographed by JOEL JSM-5500 LV, accelerated by a voltage of 15 kV at the SEM Unit, South Valley University-Qena, Egypt.
Pollen grains from unacetolyzed anthers mounted on metallic stub holders were used for palynology research. The anthers specimens were attached to the holders with double-sided self-adhesive tape. Each sample was coated with gold using a JEOL JFC 1100 E ion sputtering equipment before being analyzed using a JEOL JSM 5400 LV scanning electron microscope at Assiut University in Egypt with an acceleration voltage of 15 kV.
2.5 Terminology
The terminology of vegetative description was according to Stearn (1983). The pollen shapes, size, and surface sculpture terminology were followed by Punt et al. (2007). The author referred to the colpus length as "terminal" when the colpus length was 0.8–0.9 times the total polar axis length and "subterminal" when it was 0.7–0.8 times of the polar axis (Kamel et al. 2019).
2.6 Isolation of endophytic fungi
Endophytic fungi were isolated from plant leaves using the method described by Rossman et al. (1998). The leaves of C. erectus were gently cleaned under running water to remove dust. After washing, leaves were divided into segments (1 cm (about 0.39 in) long and diameter with midrib). The surfaces of the segments were sterilized by immersion in 75% ethanol for one minute, 5% available chlorine sodium hypochlorite for two minutes, and another sterilization with 75% ethanol for 1 min. After three washes with sterile distilled water, the segments were dried between sterile filter sheets. Four segments were inoculated on a potato dextrose agar (PDA) plate that had received chloramphenicol treatment. The plates were incubated at 28 °C for 1–2 weeks, and the growing fungi were identified as described by Moubasher (1993).
2.7 Plant and fungal extracts preparation
The first step in making the plant extract involved washing and cleaning C. erectus leaves with distilled water. The leaves were then air-dried for four days before milling into a fine powder. Ten g of the prepared leaf powder was added to 100 mL of dist. H2O, ethanol, and methanol while shaking for 24 h. The extract solutions were filtered through Whatman No.1 filter paper for purification. The ethanol and methanol extracts could dry at room temperature until constant weight. Dimethylsulfoxide (DMSO) was used to dissolve the resultant dried residue, which was then refrigerated.
The fungal isolate (A. flavus) was cultivated on PDA medium at 28 °C for seven days to prepare a crude extract from endophytic fungi. Afterward, a 250 ml (about 8.45 oz) Erlenmeyer flask containing 50 ml (about 1.69 oz) of PDB was cultured with an 8 mm (about 0.31 in) disc of the growing culture of the fungal species and incubated for 15 days (about two weeks) at 28 °C on a rotational shaker at 160 rpm. The culture broth was filtered using Whatman filter paper after the incubation period, and the filtrate was extracted using chloroform (1:1, v/v) while being constantly shaken for 24 h. At room temperature, the organic phase evaporated. Until it reached a consistent weight, the concentrated extract was kept in complete darkness (Silva et al. 2011). The obtained crude extract was DMSO-dissolved and refrigerated.
2.8 Preparation of spore suspension
The three fungal strains, Geotrichum candidum (Accession number: OL960606), Neoscytalidium dimidiatum (OL960610), and Scopulariopsis coprophila (OL960621) tested in this study were isolated in our laboratory (Applied and Environmental Microbiology, South Valley University). The fungal isolates were cultured on PDA medium at 28 ºC for seven days.
Spores were collected from fresh cultures, added to 10 mL of saline water (0.90% w/v of NaCl), and vortexed for 5 min. The concentration of spores in the suspensions was counted using a hemocytometer chamber (2 × 106 spores/mL).
2.9 Microtiter plate assay
The antifungal efficacy of extracts was evaluated using a sterilized 96-well plate: microdilution method (Ivanova et al. 2013). The antifungal capability of plant extracts and an endophytic fungus (A. flavus) was evaluated using the first five rows of a microtiter plate, and the last two were used as positive and negative controls. The extract was then added in 50 µL portions to the first well in the plate row after each well received 50 µL of sterile Potato Dextrose Broth (PDB). Each of the first five rows of wells underwent a sequential dilution process. Each well was filled with a 5 µL resazurin solution and a 5 µL fungal suspension. An indication of fungus growth was resazurin. The wells appeared with a blue-colored solution, indicating the tested extract inhibition and antifungal effect. In contrast, a pale pink to a colorless solution resulted from microbial growth or the lack of inhibition. Fifty µL of PDB, five µLof resazurin, and five µLof fungal suspension were used as the positive control (used to verify the viability of the fungus culture), whereas 50 µLof PDB and five µLof resazurin were used as the negative control (used to check the sterility of the working environment and solutions). The microtiter plates were then incubated for five days at 25 ± 2 °C. The lowest concentration of extract that inhibits the development of microorganisms is known as the minimum inhibitory concentration (MIC).
2.10 Analysis of bioactive components
According to antifungal results, aqueous and crude extracts of C. erectus leaves (ethanol and methanol) and a crude extract of A. flavus were subjected to analysis for the presence of active components by high-performance liquid chromatography (HPLC) and Gas chromatography-mass spectrometry (GC–MS), respectively. The procedures of HPLC analysis were carried out according to the method of Kim et al. (2006) at the Central Lab. of Food Industries and Nutrition Institute, National Research Centre, Giza 12622, Egypt. A quick-fit conical flask held the sample, and 20 ml of 2 M NaOH was placed. After flushing the flasks with N2, stoppers were added. The pH was changed to 2 by adding 6 M HCL after the flasks had been shaken for 4 h at room temperature. The supernatant was obtained after centrifuging the contents of each flask at 5000 rpm for 10 min. Two extractions of phenolic compounds were carried out using 50 ml each of ethyl ether and ethyl acetate (1:1). After separating the organic phase and evaporating the solvent at 45 °C, the residues were re-dissolved in 2 ml of methanol and subjected to HPLC analysis.
An Agilent Technologies 1100 series liquid chromatograph outfitted with an autosampler and a diode array detector was used to conduct the HPLC analysis. The analytical column was a Phenomenex, Torrance, California, Eclipse XDB-C18 (150 × 4.6 μm; 5 µm) with a C18 guard column. The mobile phase comprised the Acetonitrile (solvent A) and 2% acetic acid in water (solvent B). The gradient program was as follows: 100% B to 85% B in 30 min, 85% B to 50% B in 20 min, 50% B to 0% B in 5 min, and 0% B to 100% B in 5 min. The flow rate was maintained at 0.8 ml/min for the entire run time of 60 min. The injection volume was 50 µl, and flavonoids, benzoic acid derivatives, and cinnamic acid derivative peaks were concurrently seen at 360 nm and 280 nm, respectively. Before injection, all materials were filtered with an Acrodisc 0.45 µm syringe filter from Gelman Laboratory in Michigan. Peaks were located by matching UV spectra and retention times, then contrasted with the standards. The concentrations were calculated by proportional analysis (external standardization technique), comparing the peak area (PA) of the analyte in the unknown to the peak area of a known amount (C) of the standard:
The crude extract of A. flavus was screened in the Department of Chemistry, Annex (B), Faculty of Science, Assiut University, Assiut 71,516, Egypt. The Thermo Scientific TRACE GC UltraTM gas chromatograph was used to conduct endophytic fungal extract analyses. An MS Polaris QQuadrupole Ion Trap (Thermo Electron) was coupled to a J & W Scientific Fisons, Folsom, CA, bonded silica column VB5 (5% phenyl, 95% methylpolyxiloxane, 30 m at 0.25 mm internal diameter, 0.25 µm film thickness). The injector was run at 250 °C, while the interface was run at 300 °C. The following settings were entered into the oven's controller: 50 °C to 250 °C in (4 ºC/min). A 1 ml/min flow of helium served as the carrier gas. In split mode (1:20), the sample volume (1 μl) was injected. The mass spectrometer settings were EI = 70 eV, and mass range = 10–350 amu. Their identities were confirmed by comparing the retention periods and mass spectra of the extracts of endophytic fungus components to those of verified samples (analytical standards from a data database) (Bayoub et al. 2010) comparing the retention periods and mass spectra of the extracts of endophytic fungus components to those of verified samples (analytical standards from a data database) (Bayoub et al. 2010), their identities were confirmed.
3 Results
3.1 Structural observations
Conocarpus erectus was glabrous to a few hairy evergreen shrub to tree; the stem was brown with a convex and triangular form. The indumentum on the leaves were few, the leaves were appressed pubescent earlier, then glabrescent, and the trichomes were less on the adaxial surface; leaves were light green, alternate; lamina lanceolate (Fig. 1, a), with two prominent nectarous glands basally (Fig. 1, d & e); glands bowl-shaped, papillate (Fig. 2, a & b); lamina size 6.2–9.1 × 1.4–2.2 cm [L = 3.6–5.3 times W] (Fig. 1, b); margin entire to integerrimus; apex acute; base attenuate, petiolate or subsessile; petiole 2–5 mm long; ebracteate to bracteate; cauline leaves commonly with minute bracts (Fig. 1, c); bracts were lanceolate, short, hairy on both surfaces and 1–4 mm long Table 1.
Simple and glandular hairs covered the leaves. Simple trichomes were sparse, slender-shaped, appressed, and progressed from a swelling unicellular base (Figs. 2c, and 3, e). Glandular hairs (Fig. 2, d) are seen on the adaxial surface of leaves; their heads were circular or capitate-shaped, unicellular, or multicellular, and submerged within short stalks on the epidermal cells (Fig. 4, c & d). The leaves were amphistomatal, with hyphae of endophytic fungi visible on both leaf surfaces (Fig. 3); the stomata pore was short, narrowly elliptic to ovate, with no rims surrounding the pore (Fig. 2 c and d); and the stomata complex size was 26.6–37.5 × 17.3–25.0 µm Table 1.
The leaf transverse section, as shown in Fig. 4a, was composed of a layer of epidermal cells; it is 12.5–20.0 × 10.0–20.0 µm, covered with a normal layer of the cuticle (c. 5.0 µm thick), the cells of the epidermis are cubic-shaped or elongated and have been oriented perpendicular to or parallel to the section direction (Fig. 4, c, d, and e). The margins are made up of palisade and spongy tissues; the palisade layer on the adaxial surface was two rows thick, 80–125 µm thick, and thinner on the abaxial surface (one row of palisade cells, 30.0–37.5 µm thick); the spongy tissue included druses; its thickness was 130.0–232.5 µm, and it intermixed with vascular masses, which consisted of a few xylem vessels and some phloem and had a diameter of 32.5–57.5 µm. The main vascular tissue was a semi-cylinder or an arc of xylem rows and inner phloem (Fig. 4, b); the xylem rows number ranged from 11–15; each xylem row was of 1–4 vessels, and the xylem region size was 400–430 × 60–80 µm; the innermost phloem thickness was 27.5–42.5 µm. The midrib's total thickness was 750–800 µm, with dispersed druses, particularly on the abaxial surface (Fig. 5, c). Table 3 indicated the presence of phenolic compounds and idioblasts in C. erectus leaf tissues; Fig. 5c showed the phenolic-colored epidermal cells on leaf surfaces, and Fig. 5d showed a blue color accumulated in the palisade tissue.
The stem transverse section outline was triangular, 2.25–2.30 × 1.72–1.75 mm size; the epidermis was a single layer of radially elongated cells, 15.0–22.5 × 7.5–12.5 µm size, covered by a normal-thickened cuticle layer and simple trichomes are arisen (Figs. 6 and 5, a); the cortex was of 8–11 horizontal rows of parenchyma cells, with scattered druses, 112.5–200.0 µm thick at the circumference of the section; endophytic fungi mycelia are observed running through the parenchyma of the cortex (Fig. 6, c); cortical tissue at two lower corners of the sector was more thickened and with more druses (Fig. 5, b), corner-cortex was 390.0–462.5 µm thick (Fig. 6, a); vascular tissues were continuous as a complete cylinder; Secondary xylem elements consist of xylem vessel rows, these arranged singly or in nearby 2–4 aggregates; vessels number in a single xylem row was 1–3, more (4–9 vessels) in the aggregated ones (Fig. 6, b); xylem region thickness was 62.5–1.62.5 µm in Table 2, vessels were intermixed with fibres at the secondary growth part and with parenchyma at the innermost primary growth part; the phloem was in two regions, the outermost and the innermost; druses are also formed in the phloem. The outer phloem was strip-shaped, with a thickness of 30.0–62.5 µm, while masses/patches of the inner phloem were smaller in diameter, 25.0–30.0 µm diam.; the pith was centered, started by the ending border outline of the vascular tissues; it consisted of normal storage parenchyma cells, 1.27–1.37 × 0.82–0.87 mm size, with druses distributed through it. Figure 5 shows the colored phenolic compounds that accumulated in the epidermis and hypodermis cells of the C. erectus stem. Table 3 summarises the appearance of these compounds and other idioblasts (druses).
Pollens were monads; dimensions of the polar and equatorial axes and aperture size were measured and illustrated in minimum–maximum (average) values. Pollen small: P = 12.9–18.66 (15.85) µm, E = 9.0–12.58 (10.73) µm; isopolar, radially symmetric; outline: triangular-circular-6-lobate in polar view, broadly elliptic in equatorial view with flattened and slightly emarginate ends (Fig. 7, a & b); subprolate to prolate, P/E = 1.20–2 (1.52). Table 1 showed that the grains were 6-heterocolpate (Fig. 7, c), in which three simple colpi alternated with three compound ones, elongate endoaperture were slightly observed (Fig. 7, d), endoaperture size was 2.97–3.67 × 1.25–2.0 µm [L x W]. Colpi were linear and rhomboidal (in wider colpi) with acute to rounded ends, 10.86–14.82 (12.79) µm length, and varied in width, 0.34–2.58 (0.97) µm; three terminal colpi represented 0.8–0.96 times of the polar axis alternated with shorter, subterminal three ones, represented 0.8 (0.77–0.79 times of the P-axis); a bridge was observed from sexinous protrusions (Fig. 7, d); apocolpium narrow (Fig. 7, e), 4.13–7.5 µm in diameter; Fig. 7e & f illustrated the granulated colpi membrane, margin psilate to rugulate; tectum psilate to rugulate; scarce perforations in the mesocolpium were observed (Table 1).
3.2 Collection of endophytic fungi
Aspergillus flavus was isolated from healthy leaves of C. erectus.
3.3 Microtiter plate test
A microtiter plate assay was performed to detect the activity of three extracts of C. erectus and an extract of an endophytic fungus (A. flavus) in terms of MIC. The MIC of the extract is the minimum concentration required for the inhibition of fungal growth. The values of MIC for different extracts of C. erectus and A. flavus against opportunistic fungi, Geotrichum candidum, Neoscytalidium dimidiatum, and Scopulariopsis coprophila are shown in Fig. 8.
Figure 8a showed the bioassay results against Geotrichum candidum. The results revealed that extracts 2, 3, and A. flavus inhibited the growth of the yeast at a MIC of 1.25 × 103 µg/mL. On the other hand, fluconazole exhibited antifungal activity and inhibited mycelial growth at a MIC of 0.31 × 103 µg/mL. It was noted that the resazurin dye indicator was reduced in two stages, from resazurin to resorufin to hydroresorufin, which appeared colorless.
Figure 8b: Extracts 2 and 3 displayed the same antifungal ability against Neoscytalidium dimidiatum, which exhibited a MIC1.25 × 103 µg/mL. Extracts of A. flavus and fluconazole showed identical antifungal activity and inhibited mycelial growth at a MIC of 0.63 × 103 µg/mL.
Figure 8b: Scopulariopsi scoprophila was sensitive to Extract 2, which exhibited antifungal activity at a MIC of 1.25 × 103 µg/mL. Extract 3 and A. flavus showed the same antifungal activity and displayed MIC of 2.5 × 103 µg/mL. Fluconazole inhibited the growth of this fungus at a MIC of 0.31 × 103 µg/mL.
From the above results, powdered Extract 2 extracted by 70% ethanol and A. flavus extracted by chloroform were the best natural products to potentiate the antifungal activity against the tested fungi.
3.4 Determination of bioactive secondary metabolites
A crude extract of C. erectus leaves was screened by HPLC to determine the bioactive materials with antimicrobial properties (Fig. 9). Sixteen compounds were detected in the crude extract at different concentrations. Gallic acid, rutin, and rosmarinic acid were the major components represented by 3230.30, 1013.68, and 120.55 μg/g Table 4.
Kaempferol, sinapic, and apigenin were identified in the extract of C. erectus leaves with 68.51, 20.55, and 24.51 μg/g.
Protocatechuic, syringic, quercetin, and catechin were found in the extract of C. erectus leaves at 17.00, 16.58, 16.02, 9.95 μg/g, respectively.
p-hydroxybenzoic, Ferulic, Vanillic, Chrysin, and Cinnamic with 8.06, 5.54, 4.38, 2.66, and 1.89 μg/g, respectively, were recorded in the crude extract of C. erectus leaves.
Endophytic fungi A. flavus was isolated from the medicinal plant C. erectus. The endophytic fungus was cultured on 100 ml PDB media in 1 L Erlenmeyer flasks for three weeks. The culture of the fungus was extracted with chloroform. GC–MS analyzed the chloroform crude extract to determine the chemical metabolite content. Based on the results obtained from the analysis, A. flavus was noted to produce 44 bioactive components (Fig. 10). It produced a significant compound, kojic acid, with the highest content of 98.551%, which is known to exhibit antimicrobial activity. From the obtained results, 15 compounds were observed to possess antimicrobial activity: 1,2-Benzenedicarboxylicacid,bis(2-ethylhexyl)ester, 2-(2-Aminopropyl)phenol, 4-(2-Amino-1-hydroxypropyl)phenol, 9-Hexacosene, Actinobolin, Bis(2-ethylhexyl) phthalate, Cis-Aconitic anhydride, Decane, Dl-Mevalonic acid lactone, Dl-Phenylephrine, Dotriacontane, E-14-Hexadecenal, n-Octadecane, Tetracosane, and Trans-Limonene Oxide, with values fluctuating between 0.001–0.294% (Table 5).
4 Discussion
The evergreen shrub or tree known as the buttonwood (Conocarpus erectus L.) can be found in Egypt. Stace (2007) also describes it as like or found in mangroves. Two bowl-shaped nectarous glands are located at the leaf's base. These two glands were first observed on the petiole of C. erectus by Elias (1983) and Koptur (1992). A pair of elongated, swollen, hollow nectaries was discovered by Diaz-Castelazo et al. (2005) on both sides of the leaf petiole of C. erectus. In C. lancifolius, however, they were discovered by Redha et al. (2011) due to a pair of nectaries located outside of the flowers on the petiole.
In this study, there were two distinct kinds of indumentum leaves. These trichomes were both glandular and non-glandular (non-secretory). Non-glandular hairs were sparsely distributed, unicellular (simple), long, and pointed. This species was determined by a decreased trichome density, particularly in the long unicellular hairs (Almutawa and Roeland 2022). Combretaceous hairs were recognized by Stace (2007). The capitate-multicellular or circular-unicellular head of the glandular type was present, with a short and unicellular stalk. It is well-recognized that these trichomes serve defensive functions (Wagner 1991). The authors here investigated some of the non- and glandular hair forms seen in the Combretaceae family, as described by Stace (1969, 2007) and Tilney (2002).
In this research, C. erectus had amphistomatal leaves. The stomata were also discovered by Keating (1984) on the adaxial and abaxial leaf surfaces. C. lancifolius, a varied species, possesses amphistomatous leaves (Redha et al. 2011 and Al-Edany and Al-Rubaie 2012). Stomata in this study were huge, measuring 37.5 × 25.0 µm, larger stomata in mangrove leaves were found to be more capable of adjusting to challenging environmental factors like salinity, according to Sternberg and Manganiello (2014). The stoma pore was observed to be narrow and short. The decrease in stomata opening was interpreted by Tomlinson (2016). He discussed that the elaboration of cuticle extension over the pore leads to decreasing pore size. That represents adaptive behavior in mangrove leaves.
Pollen grains in this study were monads, small, isopolar, and 6-heterocolpate. Some sexinous protrusions at the colpi borders formed a bridge. Namjoyan et al. (2020) discovered the same polarity and aperture type outcomes in C. erectus grains, with a bridge established through its endoaperture. Our results reached what they were finding. Erdtman (1952) indicated that heterocolpate pollen grains are detected in some species of the family Combretaceae. Other Myrtales families have also been found to have heterocolpate grains (Dahlgren and Thorne 1984). However, Stace (2007) mentioned this crucial trait, which helped little in understanding the generic relationships in the Combretaceae family.
The main anatomical structure of the stem and leaf of the buttonwood plant was like that of most dicots. Our observations agree with El-Mahrouk et al. (2010), who described the internal stem and leaf structure. Here, a normal-thickened cuticle layer covered both epidermal cells in the stem and leaf sections of C. erectus. This layer covered epidermal cells and introduced a defense against physiological and/or metabolic problems (Korndörfe 2006). The cuticle thickness can also reflect the solar rays’ incident on the leaf. However, this structure was insignificant against the radiation because the leaf cuticle reflects only a small part of the incident radiation (Valeriano 2003).
Most parenchyma cells in our C. erectus stem and leaf anatomical structures were idioblasts. Idioblastic cells like oil, mucilage, crystalliferous, and tannin cells were found to occur in mangrove leaves (Tomlinson 2016). Idioblasts were defined as those cells that markedly differ in form, size, or content from neighboring cells (Castro and Demarco 2008). In this study, C. erectus had idioblasts of many crytalliferous druse-shaped cells. Fahn and Cutler (1992) revealed that the druses contributed to the light reflection and guiding inside the leaf. Moreover, often, druses optimize the photosynthetic performance.
Here, phenolic chemicals are present in the C. erectus stem and leaf. Different tissues, including the epidermis, hypodermis, cortex, palisade, and mesophyll, contained them in their cells and vacuoles. Different genera were reported to have these substances in their vacuoles (Alves 2006). In the ground tissue of stems of other families like Apocynaceae, Crassulaceae, Cyperaceae, etc., idioblasts secreting phenolic compounds were observed (Castro and Demarco 2008). In the leaves of other mangroves, such as Laguncularia racemosa, Lucena et al. (2011) found the phenolic compounds accumulating in the palisade cells.
The species of endophytes found in the interior tissues of C. erectus was Aspergillus flavus. Light microscopy techniques were used on stem and leaf sections to observe it. Using such microscopy tools by earlier authors (Salgado et al. 2007, Bernardi-Wenzel et al. 2010, and Verma et al. 2012) helped characterize the diversity of endofungal species.
In medicine, natural compounds are particularly important in the fight against many infections. According to Khalil et al. (2020), C. erectus is one of the species used to cure various illnesses, including fever, diabetes, catarrh, cancer, diarrhea, and others. Hussein (2016) examined the antibacterial effects of the plant's leaves on the bacteria Enterobacter, Streptococcus, E. coli, and S. aurous. In this study, several compounds were determined in C. erectus extracts and reported to have antimicrobial efficiency.
In our results, gallic acid, quercetin, rutin, rosmarinic acid, kaempferol, quercetin, sinapic, and apigenin were observed in the extracts of C. erectus. Gallic acid was demonstrated by Rajamanickam et al. (2019) to be effective against the pathogens Mannheimia hemolytica and Pasteurella multocida. Oliveiraa et al. (2016) demonstrated that quercetin and rutin were effective antifungal drugs when coupled with amphotericin B, allowing for a reduction in the amphotericin dose while increasing antifungal efficacy and minimizing side effects. Rosmarinic acid demonstrated antifungal activity against 11 Candida strains, according to Ivanov et al. (2022). Although rosmarinic acid did not bind to ergosterol, it had antifungal effects such as decreased mitochondrial activity, changed membrane integrity, and mildly suppressed protease synthesis. Antibiofilm activities include reducing exopolysaccharide synthesis, eradicating established biofilms, and preventing Candida adhesion. Rocha et al. (2019) reported that kaempferol and quercetin decreased (P < 0.05) the biomass and metabolic activity of growing biofilms of the C. parapsilosis complex. Parvu et al. (2015) reported that sinapic acid 0.660 µgml−1 was present in the extract of Hederahelix L., which showed antifungal properties against A. niger, B. cinerea, S. sclerotiorum, F. oxysporum f. sp. tulipae, and P. gladioli.
Lee et al. (2018) concluded that apigenin, extracted from the Aster yomena plant, acted as an antifungal agent by disrupting cell membranes, inducing cell shrinkage, and disrupting membranes' ability to maintain osmotic balance. Additionally, apigenin's suppression of C. albicans biofilm formation may result in additional membrane alterations.
Protocatechuic acid, catechin, and syringic acid were detected in this study. Nguyen et al. (2015) have shown Protocatechuic acid and syringic acid to have antifungal action against B. cinerea and Rhizoctonia solani. To suppress any apparent mycelial growth in both B. cinerea and R. solani, PCA was shown to have a minimum inhibitory dosage of 64 mg ml−1. Most B. cinerea conidia were injured, had altered shape, or failed to germinate after 50 and 100 mg ml−1 treatment. Syringic acid was shown to be the most fungitoxic compound: 0.5 mg/ml fully killed Ganoderma boninense, while 1.0 mg/ml only reduced the growth of the pathogen. Kumar et al. investigated catechin derivatives' antimicrobial properties (2015). For both gram-negative and gram-positive bacteria, it demonstrated excellent antibacterial action.
Based on this research, we found that endophytic fungi isolated from medicinal plants could produce natural products. We have determined kojic acid with the highest value of 98.551% and other bioactive compounds from endophytic fungi A. flavus isolated from C. erectus. Aspergillus species are commonly known to produce kojic acid with other metabolites (Bentley 2006). It is noted that this strain of A. flavus produces only kojic acid, aflatoxins and other common metabolites of Aspergillus, such as cylopiazonic acid, which were not detected in a study by Ola et al. (2019).
This study determined 1,2-benzenedicarboxylic acid, bis(2-ethylhexyl) ester, and 9-hexacosene in the crud extract of A. flavus. Kavitha et al. (2010) suggested that 1,2-benzenedicarboxylic acid, butyl 2-ethylhexyl ester play a key role in antimicrobial activity. 9-hexacosene (alpha-olefin) was analyzed from the two major compounds achieved from Jatropha zeyheri Sond. roots, which showed antimicrobial effects (Mongalo et al. 2019).
Actinobolin was recorded at 0.004% in this study. Actinobolin was evaluated by Armstrong and Hunt (1972) as an inhibitory agent against pure bacterial cultures: Bacteroides melaninogenicus, Fusobacterium fusiforme, Leptotrichia buccalis, and Veillonella parvula. These observations suggest that actinobolin may be effective in treating periodontal disease.
Bis(2-ethylhexyl) phthalate and decane were recorded in this study with 0.294 and 0.010%, respectively. Di-(2-Ethylhexyl) Phthalate was extracted from Aspergillus awamori and exhibited activity against the Candida albicans fungus and the Gram-positive bacteria Sarcinalutea. DEHP also showed cytotoxic activity against some carcinoma cell lines, as reported by Lotfy et al. (2018). Decane was extracted from the essential oil of Artemisia aucheri seed and showed activity against Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes, as Asghari et al. (2012) suggested.
Extract of Lasiodiplodia pseudotheobromae showed antibacterial activity and contained bioactive components like dl-Mevalonic acid lactone and methyl (Chaithra et al. 2020); this result agreed with our study, which indicated that crude extract of A. flavus showed dl-Mevalonic acid lactone at 0.022%.
Dl-Phenylephrine, dotriacontane, and E-14-Hexadecenal were determined to be 0.002, 0.027, and 0.030% in the crude extract of A. flavus. The different concentrations of phenylephrine exhibited antibacterial activity against bacteria such as Klebsiella pneumoniae and Staphylococcus aureus, as indicated by Zin et al. (2019). Asong et al. (2019) found that petroleum ether and 50% methanol extracts from four medicinal plants showed antimicrobial activity against six microbes: Bacillus cereus, Shigella flexneri, Candida glabrata, Candida krusei, Trichophyton rubrum, and Trichophyton tonsurans, using the micro-dilution test. GC–MS analysis revealed the presence of dotriacontane as a bioactive component. After being analyzed with GC–MS, Abraham et al. (2018) revealed that crude extracts of Pseudarthria viscida and its endophytes have E-14-Hexadecenaland (with). Most of the compounds in the extracts were identified as possessing therapeutic properties.
N-Octadecane and tetracosane were represented by 0.032 and 0.034% in the crude extract of A. flavus, respectively. Based on the analysis of GCSP, n-octadecane (30.5%) was detected in the bulb oil of Allium nigrum L. that exhibited antimicrobial activity, especially against Enterococcus faecalis and Staphylococcus aureus (Rouis-Soussi et al. 2014). Allium tripedale extract had a considerable inhibitory effect against various Candida species, and among the major components of the extract was tetracosane.
In conclusion, this study documented the morphological characteristics and internal anatomy of the stems and leaves of Conocarpus erectus L. and introduced palynological information. The morpho-anatomical attributes considered the structural arrangement of plant tissues, phytochemicals, and endophytes. The antifungal activity of plant extracts and their endophytes resulted from active metabolites such as kojic acid, gallic acid, rutin, and rosmarinic acid.
Data availability
Data were available in the manuscript and at NCBI.
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The Institute Ethics Committee approved the study protocol. Approval for fungal isolates in this study was granted by theResearch Ethics Committee of Faculty of Science, South Valley University, Egypt (No.: 001/11/22).
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Hussein, N.R.A., El-Dawy, E.G.A.M. Morpho-anatomical attributes of the Egyptian Conocarpus erectus L. (Combretaceae R.Br.) with its phytochemicals and fungal-endophytes. Symbiosis 92, 91–109 (2024). https://doi.org/10.1007/s13199-023-00960-6
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DOI: https://doi.org/10.1007/s13199-023-00960-6