Abstract
The small molecule characteristics and nutritional value of egg white hydrolysates have been widely used. In the present study, in vitro and in vivo models were used to investigate the hepatoprotective effect of egg protein hydrolysate (EWH) by regulating the expression of antioxidant enzymes. The in vitro experiment results showed that 0.1, 0.5, and 1 mg/mL of EWH enhanced antioxidant activity in HepG2 cells by increased glutathione peroxidase (GPx) activity and reduced glutathione (GSH) levels. The in vivo experiment results showed that EWH (L) (38.5 mg/kg BW) and EWH (H) (385 mg/kg BW) alleviated carbon tetrachloride (CCl4)-induced hepatotoxicity in SD rats through reduced levels of serum aspartate aminotransferase (AST) alanine aminotransferase (ALT), and lipid peroxidation products malondialdehyde (MDA). In addition, EWH also ameliorates CCl4-induced hepatotoxicity in SD rats by increasing the antioxidant activity of GSH levels with a decrease in oxidized glutathione (GSSG) levels. Besides, EWH ameliorates liver tissue injuries by CCl4-induction. EWH has the highest glutamic acid in free amino acid composition, the second highest was aspartic acid, and the third was cystine, 204, 141, and 125 mg/100 g, respectively. These results suggest EWH has hepatoprotective potential through reduced lipid peroxidation products and enhanced antioxidant activity.
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The datasets generated and/or analyzed during the current study are not publicly available due [REASON WHY DATA ARE NOT PUBLIC] but are available from the corresponding author at reasonable request.
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Abbreviations
- GPx:
-
Glutathione peroxidase
- GSH:
-
Reduced glutathione
- CCl4 :
-
Carbon tetrachloride
- AST:
-
Serum aspartate aminotransferase
- ALT:
-
Alanine aminotransferase
- MDA:
-
Malondialdehyde
- GSSG:
-
Oxidized glutathione
- ROS:
-
Reactive oxygen species
- DMEM:
-
Dulbecco’s modified eagle medium
- PBS:
-
Phosphate-buffered saline
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The authors are grateful to appreciate the invaluable support of the Ministry of Science and Technology of Taiwan in this study.
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This study was funded with the support of the Ministry of Science and Technology of Taiwan (Grant No. MOST 103-2815-C-022-006-B).
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Conceptualization, Y-TC, and C-WT; methodology, Y-TC, and Y-AC; validation, C-YH, R-QX, and C-HK; formal analysis, C-WT, and C-YH; investigation, C-HK, and C-CW; resources, Y-AC, and R-QX; data curation, Y-TC; writing-original draft preparation, Y-TC; writing-review and editing, C-YH, C-CW and S-LH; supervision, S-LH; project administration, S-LH All authors read and agreed to the published version of the manuscript.
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Chen, YT., Tu, CW., Hou, CY. et al. Evaluation of egg white hydrolysates on the hepatoprotective effect in vitro and in vivo. J Food Sci Technol 60, 1633–1641 (2023). https://doi.org/10.1007/s13197-023-05706-2
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DOI: https://doi.org/10.1007/s13197-023-05706-2